相关实验视频

Updated: Jun 6, 2025

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair

Published on: September 7, 2017

显示TGF-β1的基因工程细菌细胞在3D微环境中促进血管生成

In-Hyuk Baek^1,2, Volkhard Helms², Youngjun Kim¹

¹Environmental Safety Group, Korea Institute of Science & Technology Europe GmbH, Campus E71, 66123 Saarbrücken, Germany.

Journal of functional biomaterials

|November 26, 2024

概括

显示TGF-β1的工程菌体在3D培养中持续刺激内皮细胞迁移和管形成,模仿细胞外基质 (ECM) 增强血管生成. 这表明它们作为组织工程和体外模型的生物材料的潜力.

科学领域:

生物材料科学生物材料科学
组织工程是组织工程.
细胞生物学细胞生物学

背景情况:

线状细菌体是多功能纳米材料,在药物输送和组织工程中具有应用.
转基因菌体可以针对特定的组织,如瘤,进行治疗.
结合微环境模拟系统的3D细胞培养试验对于药物毒性查至关重要.

研究的目的:

为了研究具有TGF-β1表现的工程细菌体在体外促进血管生成的潜力.
为了评估TGF-β1显示细菌体在微环境中作为持续刺激剂的有效性.
探索功能化菌体作为生物材料在生物医学工程中的应用.

主要方法:

显示TGF-β1的工程细菌被用于微环境模拟系统中的3D细胞培养试验.
检测包括RT-qPCR,ELISA和光显微镜,以确认血管性标记物表达 (例如CD31).
微流体通道进行了表面处理,以调节3D血管性结构的形成.

主要成果:

显示TGF-β1的菌体显著增强了细胞内膜细胞迁移和细胞外基质 (ECM) 内的管形成.
菌体作为连续刺激剂,在促进血管生成方面表现优于单独的TGF-β1或非修饰菌体.
确认了血管新生标记的表达和3D血管新生结构的形成,这表明TGF-β1充当了化学反应因子.

结论:

生物相容的TGF-β1显示细菌菌体可以持续刺激血管新生模型的体外微环境.
功能化的菌体显示出作为生物医学工程应用的多功能生物材料的潜力.
这一策略可用于在体外测试中开发用于组织工程的血管生成矩阵.

关键词:

这是一个ECM,ECM是ECM.在HUVECs中,血管新生是因为血管新生.细菌体显示显示器有线细菌的细菌.芯片上的实验室

更多相关视频

Live Cell Imaging of the TGF- β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System

Live Cell Imaging of the TGF- β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System

Published on: July 30, 2018

Spheroid Assay to Measure TGF-β-induced Invasion

Spheroid Assay to Measure TGF-β-induced Invasion

Published on: November 16, 2011

相关概念视频

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Mechanism of Angiogenesis

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...