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无标签的光学传输断层扫描用于直接的真菌学检查和细胞内动力学的监测

Eliott Teston¹, Marc Sautour^2,3, Léa Boulnois⁴

¹Lipness Team, Translational Research Center in Molecular Medicine- INSERM Joint Research Unit (CTM-UMR1231), University of Burgundy, 21000 Dijon, France.

Journal of fungi (Basel, Switzerland)

|November 26, 2024

概括

动态全场光传输断层扫描 (D-FF-OTT) 可实现无标签的,高分辨率的真菌活细胞成像. 这一突破有助于通过可视化真菌结构和细胞内动态来诊断侵入性真菌感染.

科学领域:

生物物理学的生物物理.
菌类学菌类学是指菌类学.
医疗成像医学成像

背景情况:

活细胞成像通常依赖于光标签.
目前的方法可能不适合直接的真菌学检查.
侵入性真菌感染需要快速准确的诊断.

研究的目的:

为无标签的活细胞真菌成像引入动态全场光传输断层扫描 (D-FF-OTT).
评估D-FF-OTT对真菌结构高分辨率成像的能力.
探索D-FF-OTT用于监测真菌中的细胞内过程.

主要方法:

动态全场光传输断层扫描 (D-FF-OTT) 的应用.
包括 *Candida albicans*, *Aspergillus fumigatus* 和 *Rhizopus arrhizus* 在内的真菌物种的成像.
没有标签的,高分辨率的活细胞显微镜.

主要成果:

D-FF-OTT精确地检查了基本的真菌结构.
该技术确定了负责感染的关键真菌物种.
细胞内有机体和囊泡的细胞内贩运在活的真菌中被可视化.

结论:

D-FF-OTT是第一个基于光干扰的技术,用于直接的真菌学检查.
这种方法提供高分辨率,无标签的真菌活细胞成像.
D-FF-OTT为快速的真菌感染诊断开辟了新的视角.

关键词:

的新陈代谢是真菌的新陈代谢.侵袭性真菌感染侵袭性真菌感染活细胞成像技术使用.医学真菌学医学真菌学光学断层扫描 (光学断层扫描) 是一种光学断层扫描.

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