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遵循路径:揭示一种耐醇 Candida parapsilosis 疫情通过FTIR光谱和STR分析

Elena De Carolis¹, Carlotta Magrì¹, Giulio Camarlinghi²

¹Dipartimento di Scienze di Laboratorio ed Ematologiche, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, 00168 Roma, Italy.

Journal of fungi (Basel, Switzerland)

|November 26, 2024

概括

使用富里埃变换红外光谱仪 (FTIR) 快速识别耐可纳的Candida parapsilosis,有助于管理与医疗相关的感染. 这种方法与遗传分析相结合,有助于追踪疫情并实施有效的控制策略.

科学领域:

医学微生物学医学微生物学
传染性疾病传染性疾病
频谱学是一种光谱学.

背景情况:

坎迪达 (Candida parapsilosis) 是一个重要的机会性病原体,导致与医疗相关的感染.
准确的鉴定和基因定型对于管理疫情和感染控制至关重要.
在意大利的托斯卡纳康复诊所 (CRT) 发生了一次耐富可纳的C. parapsilosis疫情.

研究的目的:

为了追踪在CRT设施的抗可纳的Candida parapsilosis疫情.
评估福里埃变换红外光谱 (FTIR) 和短串重复 (STR) 分析在疫情调查中的实用性.
为了将FTIR资料与遗传突变和醇耐药水平相关联.

主要方法:

在疫情爆发期间采集了24个临床样本和2个环境样本.
应用FTIR光谱学进行隔离分析.
对ERG11基因的基因测序和STR类型的基因定型.

主要成果:

在FTIR分析中,分离物被分为两个主要组.
这些与与耐药性相关的ERG11突变 (Y132F,R398I),醇耐药性和隔离年相关.
结合FTIR和STR类型识别确定了基因相似的分离集群.

结论:

与STR类型结合使用FTIR光谱,提供了一种全面的方法来识别和跟踪抗可纳的C. parapsilosis.
实时FTIR分析可以为目标感染控制策略提供信息.
FTIR是管理由C. parapsilosis引起的医院感染的一个有价值的工具.

关键词:

在Candida parapsilosis中使用.在FTIR光谱学中使用.简短的同时重复重复.耐亚醇耐药性耐亚醇耐药性医院内感染医院内感染疫情爆发管理管理

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