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运输活动的UV/H₂O₂-退化Fucoidans及其结构特征

Biyang Zhu^1,2, Jiacheng Wang¹, Lijun You^1,2,3

¹School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.

|November 26, 2024

概括

具有较低分子量的降解后的福科丹 (DFs) 显示出增强的化能力. 这些富可伊丹-合物改善了肠道的吸收,并保护了肠道屏障,为补充剂提供了一种新的方法.

科学领域:

生物化学生物化学
材料科学材料科学材料科学
营养科学营养科学

背景情况:

聚糖被探索作为补充剂.
富科丹是海洋多糖类,具有潜在的生物活性.
优化结合和吸收对于补充剂的有效性至关重要.

研究的目的:

为了研究分子质量对化能力和降解的福科丹 (DFs) 的肠道吸收的影响.
探索DFs结合和运输增强的潜在机制.
评估DF-酸作为改进的补充剂的潜力.

主要方法:

紫外线/H2O2处理以降解fucoidan并获得具有不同分子量 (Mws) 的DF.
评估结能力和碳酸含量.
对 DFs 的形态分析.
对DF-相互作用的热力学分析.
使用Caco-2细胞单层的体外肠道输送研究.
评价上皮质屏障完整性和紧结蛋白表达.

主要成果:

随着Mw的下降,DF的化能力增加,这归因于自由基降解导致的碳素含量增加.
DF形状从棒状转变为更短,更软的链条.
DF-结合是自发的,由静电相互作用驱动.
较低的MW DF-合物表现出通过Caco-2细胞的增强运输和加快流入.
通过减轻紧密结节蛋白损伤,DF-酸保护了上皮屏障.

结论:

通过自由基介导的降解提供了一种有效的方法来增强福柯丹的化特性.
低分子量降解的富可伊丹-酸盐表现出优越的肠道运输和吸收能力.
这些发现支持开发基于多糖的先进补充剂,改善生物可用性和肠道健康益处.

关键词:

补充剂补充剂化化化化降解降解降解降解降解.这就是Fucoidan.交通运输运输运输运输运输运输运输运输运输

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