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循环亚酸细胞毒性的综合方法使用In Vitro (2D和3D模型) 和In Silico方法

Carmen Martínez-Alonso¹, Luana Izzo², Yelko Rodríguez-Carrasco¹

¹Department of Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine, Faculty of Pharmacy and Food Science, University of Valencia, Av. Vicent A Estelles s/n, Burjassot, 46100 Valencia, Spain.

|November 26, 2024

概括

环酸 (CPA) 是一种在食物中发现的强有力的神经毒素. 这项研究揭示了CPA随时间和剂量增加细胞毒性,影响人类神经母细胞瘤细胞并预测血脑屏障的透.

科学领域:

神经科学是一个神经科学.
毒理学毒理学毒理学
菌类毒理学菌类毒理学

背景情况:

环酸 (CPA) 是一种神经毒性菌毒素,由Aspergillus和Penicillium物种产生的.
在受污染的水果,谷物和坚果中通常会发现CPA,这可能会给健康带来潜在的风险.

研究的目的:

用2D单层和3D球形来比较CPA在人类神经母细胞瘤SH-SY5Y细胞中的细胞毒性.
使用in silico模型评估CPA的毒动力学.
评估CPA对球体形态和组织的影响.

主要方法:

细胞毒性通过使用MTT测定在不同的CPA暴露时间 (24,48,72小时) 中进行评估.
在模型 (SwissADME,admetSAR) 用于ADMEt的配置文件.
在CPA暴露后观察到SH-SY5Y球体的形态变化.

主要成果:

与3D球形相比,CPA表现出剂量和时间依赖的细胞毒性,在2D单层中IC50值较低.
暴露于CPA诱导了SH-SY5Y球体中的形态变化和分离.
在分析预测CPA的高胃肠道吸收和血脑屏障透率.

结论:

3D球形模型提供了比传统的2D单层更强大的CPA细胞毒性评估.
在体中,有毒动力学分析提升了对CPA潜在的系统性影响的理解.
这种综合方法为真菌毒素风险评估提供了一个全面的策略.

关键词:

在SH-SY5Y细胞中.环亚酸环亚酸是指环亚酸中的一种.细胞毒性细胞毒性在形方法中,在形方法中.球形状的球形体是指球形体.

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