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交互网络传染性支气管炎病毒 Nsp2 与宿主蛋白质的特性

Mengmeng Wang¹, Zongyi Bo^1,2, Chengcheng Zhang¹

¹Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.

Veterinary sciences

|November 26, 2024

概括

这项研究确定了与传染性支气管炎病毒 (IBV) Nsp2蛋白相互作用的宿主蛋白,揭示了它们在病毒病原和复制中的作用. 这些发现突出了NSP2的存在.

科学领域:

兽医病毒学兽医病毒学
分子病原体的产生.
禽类的健康禽类的健康

背景情况:

传染性支气管炎病毒 (IBV) 在家禽中造成重大经济损失.
Nsp2蛋白是IBV的潜在关键毒性因子,但其宿主相互作用尚不清楚.

研究的目的:

识别和描述与IBV Nsp2蛋白相互作用的宿主蛋白质.
阐明这些相互作用在IBV病原和病毒复制中的作用.

主要方法:

酵母双混合选和分子对接模拟以确定潜在的相互作用蛋白质.
同免疫沉和共聚焦显微镜来验证相互作用.
基因本体学 (GO),KEGG和蛋白质与蛋白质相互作用 (PPI) 数据库分析.
在IBV感染期间对宿主蛋白质表达水平的分析.

主要成果:

十种宿主蛋白 (COX1,COX3,NFIA,ITGA1,ATP1B1,ATP1B3,ABCB1,ISCA1,DNAJA1,IREB2) 被确定为IBV Nsp2.2的潜在相互作用体.
与ATP1B3,DNAJA1和ISCA1的相互作用得到了验证.
相互作用的蛋白质参与ATPase激活,Fe-S集群结合,离子稳态和先天免疫.
感染IBV改变了宿主蛋白的表达,导致COX3,COX1,ATP1B1,ATP1B3的下调和NFIA,DNAJA1,IREB2.3的上调.
在IBV上调调节DNAJA1的表达,可能增强病毒复制.

结论:

IBV Nsp2蛋白与参与关键细胞功能的多种宿主蛋白相互作用.
这些相互作用可能有助于IBV病变和病毒扩散.
通过IBV对DNAJA1的升级可能是一种增强病毒复制的机制,需要进一步调查.

关键词:

DNAJA1 DNAJA1 的意思在 Nsp2 的情况下.感染性支气管炎病毒感染性支气管炎病毒分子对接的分子对接.蛋白质相互作用蛋白质相互作用酵母两种混合的酵母

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