Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Polyprotic Acids03:38

Polyprotic Acids

Acids are classified by the number of protons per molecule that they can give up in a reaction. Acids such as HCl, HNO3, and HCN that contain one ionizable hydrogen atom in each molecule are called monoprotic acids. Their reactions with water are:

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The role of community champions in place-based early years support: how can we successfully share knowledge and build parent confidence?

Perspectives in public health·2024
Same author

Stable quantum-correlated many-body states through engineered dissipation.

Science (New York, N.Y.)·2024
Same author

The impact of the World Health Organization Health Promoting Schools framework approach on diet and physical activity behaviours of adolescents in secondary schools: a systematic review.

Public health·2020
Same author

A quality improvement project to increase self-administration of medicines in an acute hospital.

International journal for quality in health care : journal of the International Society for Quality in Health Care·2018
Same author

Discharge without alarm(s)!

Journal of perinatology : official journal of the California Perinatal Association·2018
Same author

An observer-blinded randomized controlled pilot trial comparing localized immersion psoralen-ultraviolet A with localized narrowband ultraviolet B for the treatment of palmar hand eczema.

The British journal of dermatology·2017

相关实验视频

Updated: Jul 12, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

13.4K

在人体血中测量脱酸:同时通过UPLC/MS-MS直接测量酸/脱酸对.

P-C Violet1, N Munyan1, H F Luecke2

  • 1Molecular and Clinical Nutrition Section, Digestive Diseases Branch, Intramural Research Program, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA, 20892.

Redox biology
|November 26, 2024
PubMed
概括

一种新的UPLC/MS/MS方法准确地测量了人体血中的酸 (维生素C) 和脱酸. 这一突破使我们能够更好地了解维生素C在健康和疾病中的作用.

关键词:
亚斯科布酸是一种亚斯科布酸.脱水阿斯科比克酸脱水阿斯科比克酸人类血是人类的血.质谱测量质量谱测量单喷剂是一种单喷剂.维生素C 维生素C是一种

更多相关视频

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.8K
Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties Lactuca sativa L. and Crop Wild Relatives Lactuca spp.
10:22

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties Lactuca sativa L. and Crop Wild Relatives Lactuca spp.

Published on: June 30, 2020

13.4K

相关实验视频

Last Updated: Jul 12, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
11:56

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

13.4K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.8K
Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties Lactuca sativa L. and Crop Wild Relatives Lactuca spp.
10:22

Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties Lactuca sativa L. and Crop Wild Relatives Lactuca spp.

Published on: June 30, 2020

13.4K

科学领域:

  • 生物化学 生物化学
  • 分析化学 分析化学
  • 人体生理学 人体生理学

背景情况:

  • 亚酸 (AA) 和脱亚酸 (DHA) 形成了一个关键的生物氧化还原对.
  • 目前的分析方法不能同时量化AA和DHA,这阻碍了研究.
  • 在了解维生素C方面取得了进展.
  • 医学的生理和病理生理学作用受到这种分析差距的限制.

研究的目的:

  • 开发和验证一种新的UPLC/MS/MS方法,用于同时独立量化人体血中的AA和DHA.
  • 克服现有技术在测量AA/DHA对的局限性.

主要方法:

  • 采用超高性能液态染色学与并联质谱学 (UPLC/MS/MS) 的方法.
  • 用同位素标记的内部标准用于AA和DHA,以确保精度和准确性.
  • 使用了一种简化的血制备方案,只涉及两个步骤.

主要成果:

  • 该UPLC/MS/MS方法证明了高精度和可重复性,测试间变化很低 (<10%).
  • 对AA和DHA的量化下限为16nM,在100倍度范围内具有线性 (R2>0.99).
  • 对人体血样本的分析显示AA的平均度为56±14μmol/L,DHA的平均度为6±2μmol/L.

结论:

  • 这种开创性的UPLC/MS/MS方法为测量人体血中的AA/DHA对提供了强大而准确的工具.
  • 开发的方法有助于进一步研究维生素C氧化还原循环的生理和病理生理影响.