酵素修饰对人参中性多糖的结构,抗氧化活性和益生菌活性的影响
Binbin Dong1, Zong Hou2, Zhong Zheng2
1State Key Laboratory of Electroanalytical Chemistry, National Center of Mass Spectrometry in Changchun and Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 130022 Changchun, China; School of Applied Chemistry and Engineering, University of Science and Technology of China, 230026 Hefei, China.
International journal of biological macromolecules
|November 26, 2024
概括
人参中性多糖体 (GPN) 的酶性修饰产生了EGPN,显示出增强的抗氧化和神经保护作用. 此外,EGPN还改善了肠道微生物群平衡,并增加了有益的短链脂肪酸 (SCFA).
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 神经科学是一个神经科学.
背景情况:
- 人参中性多糖 (GPN) 丰富,但活性低于酸性多糖.
- 酶修饰提供了一种新的方法来增强GPN的生物活性.
研究的目的:
- 为了酶性地将GPN修改为EGPN,并评估其抗氧化,神经保护和肠道微生物群调节效应.
- 研究EGPN对PC12细胞的影响以及体外肠道发酵模型.
主要方法:
- 复合酶降解以从GPN产生EGPN.
- 使用GPC,FT-IR,XRD,泽塔潜力,TG,冷SEM和AFM对EGPN的表征.
- 在体外抗氧化剂测定,对Aβ25-35受损的PC12细胞进行神经保护研究,以及使用人类肠道微生物群进行体外发酵.
主要成果:
- EGPN在体外表现出显著的抗氧化活性,并保护PC12细胞免受Aβ25-35引起的损伤.
- 肠道微生物群的EGPN代谢增强了受损的PC12细胞的活力.
- EGPN增加了肠道微生物群的多样性,促进了短链脂肪酸 (SCFA) 的产生,减少了有害细菌 (Enterococcus,Allobaculum),并促进了有益的细菌 (Lactobacillus,Prevotella,Ruminococcus).
结论:
- 酶降解有效地增强了GPN的生物活性,产生具有强大的抗氧化和神经保护性质的EGPN.
- 通过调节肠道微生物群的组成和功能,EGPN显示出恢复肠道平衡的潜力.
- EGPN是抗氧化剂治疗,神经保护和肠道健康管理中的一个有前途的功能成分.
相关概念视频
Protein Glycosylation
6.8K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Glycosylation occurs in...
6.8K
Oligosaccharide Assembly
2.8K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
2.8K
Biosynthesis of Polysaccharides
1
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
1
Oral Hypoglycemic Agents: α-Glucosidase Inhibitors
159
α-glucosidase inhibitors, including acarbose (Precose), miglitol (Glyset), and voglibose (Voglib) (primarily available in Asia), are drugs that control blood sugar levels by delaying the digestion of starch and disaccharides. They achieve this by inhibiting α-glucosidase enzymes in the intestine, which slow the absorption of carbohydrates in the intestine, which in turn leads to a prolonged release of the glucoregulatory hormone GLP-1 from intestinal L-cells.
Acarbose and miglitol are...
Acarbose and miglitol are...
159


