穿过表皮的定向纤维动脉需要Ccdc57介导的轴膜方向和基底体极性之间的合
Xinwen Pan1,2, Chuyu Fang1,3, Chuan Shen1,2
1Key Laboratory of Multi-Cell Systems, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, Shanghai, China.
Nature communications
|November 26, 2024
概括
移动的毛通过基底脚的旋转来调整它们的节拍方向,这个过程取决于Ccdc57. 这种蛋白质确保了正确的纤毛功能,防止像小鼠的水脑这样的疾病.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 生物物理学的生物物理.
背景情况:
- 移动的乳毛表现出协调的节拍方向 (轴膜方向,AOs) 跨越上皮质,以产生方向液体流.
- 这种协调平面极性依赖于基底脚 (BF),基底体 (BB) 附属物,旋转以与AO保持一致.
- 关于BF-AO关系的确切时间和机制尚不清楚.
研究的目的:
- 为了研究基底体极化期间基底脚-轴突面指向合的机制和时间.
- 为了确定参与确定移动毛平面极性的关键蛋白质.
主要方法:
- 使用活细胞成像来观察基底体旋转和基底脚偏振.
- 采用基因操纵 (Ccdc57淘汰赛) 来评估其在状细胞对齐和功能中的作用.
- 检查了小鼠模型 (Ccdc57-/-) 以评估毛功能受损的生理后果.
主要成果:
- 已经确定,基底脚-轴膜方向合发生在基底体旋转极化过程中,并且需要Ccdc57.
- 证明Ccdc57定位为基底体上的旋转不对称的点点,偏离BF以稳定BF-AO关系.
- 观察到Ccdc57缺陷的毛缺乏BF-AO合,导致单细胞跳动失调和集体流动受损.
- 表明Ccdc57淘汰赛小鼠表现出严重的脑水和高死亡率,由于缺陷的脑脊髓液流.
结论:
- 揭示了Ccdc57在建立和维持运动的平面极性中的关键作用.
- 鉴定出Ccdc57是基底体极化过程中基底脚 - 轴膜方向合的关键调节器.
- 突出了协调状元功能的生理重要性,缺陷导致严重的发育异常和死亡率.
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