相关实验视频

Updated: Jun 21, 2026

Isolation of Tissue Extracellular Vesicles from the Liver

Isolation of Tissue Extracellular Vesicles from the Liver

Published on: August 21, 2019

细胞外囊泡的不同储存和冷协议:系统性审查

Shahin Ahmadian^1,2, Negin Jafari¹, Amin Tamadon^3,4

¹Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Stem cell research & therapy

|November 27, 2024

概括

细胞外囊泡 (EVs) 的最佳储存对于再生医学至关重要. 在-80°C的快速结和像trehalose这样的稳定剂保持了EV的完整性,而结解周期会降低它们.

科学领域:

生物技术是生物技术.
再生医学是一种再生医学.
纳米医学是一种纳米医学.

背景情况:

细胞外囊泡 (EVs) 在再生医学中表现有前途.
电动汽车的纳米尺度特性使它们对储存条件敏感.
适当的存储对于保持电动汽车的完整性和功能至关重要.

研究的目的:

系统地审查存储协议对EV特征的影响.
确定维护EV结构,分子和功能完整性的最佳条件.

主要方法:

关于储存温度,冷,冷解周期和稳定剂的综合文献搜索.
分析了50项评估EV度,大小,形态,货物和生物活性的研究.

主要成果:

快速冷和恒定的-80°C储存可保存EV数量和货物.
多个冷解周期降低度,RNA含量和生物活性,增加聚合.
稳定剂 (例如,三) 和存储在原生生物流体中可以提高EV的稳定性.

结论:

精确的EV存储协议对于可重复的研究和临床使用至关重要.
需要进行进一步的研究,以制定在各种应用中为电动汽车稳定性制定具体指南.

关键词:

结冷保存是一种冷保存.细胞外囊泡中的细胞外囊泡.诚信诚信是一种诚信.稳定的稳定性稳定的稳定性储存储存储存储存

更多相关视频

Evaluation of the Storage Stability of Extracellular Vesicles

Evaluation of the Storage Stability of Extracellular Vesicles

Published on: May 22, 2019

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

Published on: September 16, 2022

相关概念视频

Microbial Classification System

Microbial Classification System

Classification is the process of organizing organisms into hierarchically inclusive groups based on their phenotypic similarities or evolutionary relationships. A species comprises one or more strains, and closely related species are grouped into genera. Genera are further classified into families, families into orders, orders into classes, and so forth, up to the domain level, which is the broadest taxonomic rank derived from a combination of phenotypic and genotypic data.The nomenclature of...