通过面向味道的合成和微生物群落的构建,在黄葡萄酒 (黄江) 中阐明核心微生物群
Qi Peng1, Huajun Zheng1, Huihui Zhou1
1National Engineering Research Center for Chinese CRW (Branch Center), School of Life and Environmental Sciences, Shaoxing University, 900 Chengnan Road, Shaoxing 312000, China.
Food research international (Ottawa, Ont.)
|November 27, 2024
概括
这项研究确定了中国传统黄州的关键微生物和挥发性化合物. 一个合成的微生物群落成功地复制了它的味道,增加了酒精含量并降低了酸度.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 发酵技术的发酵技术
背景情况:
- 黄江酒是一种传统的中国酒精饮料,具有深厚的文化意义.
- 黄发酵的微生物生态和挥发性有机化合物 (VOC) 概况尚未完全理解.
研究的目的:
- 为了确定核心挥发性有机化合物 (VOCs) 定义黄江风味.
- 确定负责黄州发酵的关键微生物.
- 建立一个合成微生物社区,以优化黄州的生产.
主要方法:
- 头部空间固体相微提取气色谱质谱 (HS-SPME-GC-MS) 用于VOC分析.
- 芳香复制和省略实验以精确确定关键的芳香化合物.
- 用于微生物社区分析的元基因组测序和定量PCR (QPCR).
- 合成微生物社区模型的构建和测试.
主要成果:
- 确定了主要的挥发性有机化合物,包括异本醇,异醇和β-乙醇.
- 描述了微生物群落在发酵过程中的时间和空间动态.
- 合成社区模型准确地复制了黄州的核心VOC和感觉特征.
- 该模型增强了合成的黄江湖的酒精含量和降低了酸度.
结论:
- 在黄州建立了特定微生物和风味配置文件之间的联系.
- 演示了合成微生物群落控制黄江发酵的潜力.
- 提供了改善黄州生产和发酵饮料研究的基础见解.
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