通过使用CFU,RT-PCR和SEM对抗Enterococcus faecalis的PUI和XPF文件激活的甲酸盐溶液的抗菌能力的研究
Radovan Jovanović1, Jugoslav Ilić2, Ljiljana Šubarić1
1Dental Clinic, Faculty of Medicine, University of Priština in Kosovska Mitrovica, 38220 Kosovska Mitrovica, Serbia.
Bioengineering (Basel, Switzerland)
|November 27, 2024
概括
先进的灌技术,如被动超声波灌 (PUI) 和XP-endo Finisher (XPF) 显著减少了根管中的Enterococcus faecalis. 这些方法改善了超越标准协议的内牙消毒.
科学领域:
- 内牙微生物学 内牙微生物学
- 牙科治疗学 牙科治疗学
背景情况:
- 根管微生物的完全根除对于内牙治疗的成功至关重要.
- 标准灌方案可能无法完全消除细菌,包括Enterococcus faecalis,这是治疗失败的常见原因.
研究的目的:
- 评估被动超声波灌 (PUI) 和XP-endo Finisher (XPF) 在实验污染的根管中减少Enterococcus faecalis的疗效.
- 将这些先进的灌技术与标准的注射器和针灌协议进行比较.
主要方法:
- 42个根通道被实验性地用Enterococcus faecalis.alis进行了接种.
- 测试了三个灌协议:标准注射器/针,PUI和XPF激活.
- 使用实时定量聚合酶链反应 (RT-PCR) 和殖民地形成单位 (CFU) 来量化微生物减少.
主要成果:
- 无论是PUI和XPF灌方案都导致Enterococcus faecalis.的显著减少.
- 与标准灌相比,这些先进的技术显示出更高的抗微生物疗效.
结论:
- 补充灌协议,特别是PUI和XPF,显著提高根管道消毒.
- 这些方法在消除微生物方面提供了显著的改进,积极影响了内牙治疗结果.
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