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从农业工业副产品中可持续的omega-3脂质生产,使用thraustochytrids:使过程开发,优化和扩展成为可能

Guilherme Anacleto Dos Reis¹, Brigitte Sthepani Orozco Colonia¹, Walter Jose Martínez-Burgos¹

¹Department of Bioprocess Engineering and Biotechnology, Polytechnic Center, Federal University of Parana, Rua Cel. Francisco H. dos Santos-100, Curitiba 81530-000, PR, Brazil.

Foods (Basel, Switzerland)

|November 27, 2024

概括

在农业工业副产品上种植的杆菌有效地产生omega-3多不和脂肪酸 (PUFA). 这项研究利用甘糖蜜,玉米酒和酵母奶油优化了生物质和脂质产量,以实现可持续的PUFA生产.

科学领域:

生物技术是生物技术.
海洋微生物学海洋微生物学
营养科学营养科学

背景情况:

草基是omega-3多不和脂肪酸 (PUFA) 的一个主要来源.
农业工业副产品为微生物培养提供了具有成本效益的基材.
可持续的PUFAs生产对于人类和动物的营养至关重要.

研究的目的:

优化使用农业工业副产品种植的 * Schizochytrium limacinum * SR21.
为了最大限度地提高生物质和脂质的生产,以产生omega-3 PUFA.
为了确定成本效益高的基板,以可持续地种植thraustochytrid.

主要方法:

优化生物质和脂质生产在摇瓶和池生物反应器.
甘糖蜜 (SCM),玉米液 (CSL) 和剩余酵母奶油 (RYC) 作为基质的评估.
确定最佳基质度和种植参数.

主要成果:

振动瓶的优化提高了生物质,脂质含量和脂质产量的1.12,1.72和1.92倍,分别.
一个10升生物反应器实现了39.29g/L生物质,14.98g/L脂质度和32.83%的DHA.
最佳基质度 (66 g/L SCM,24.5 g/L CSL,6 g/L RYC) 产生了0.87.7的可取性指数.

结论:

农业工业的副产品是有效的低成本基质,用于培基脂的生产.
这种生物工艺为欧米茄-3 PUFA 生产提供了一个可持续的途径.
结果支持在食品强化,补充剂和纯素omega-3来源中的应用.

关键词:

DHA (docosahexaenoic acid) 是一种多可萨赫萨酸 (DHA) 的一种化合物.欧米茄-3 脂肪酸欧米茄-3 脂肪酸通过状基体的状基体适合素食主义者的omega-3来源

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