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相关概念视频

The Thyroid Gland01:23

The Thyroid Gland

The thyroid gland is a small, butterfly-shaped gland located in the neck and covers the anterior surface of the trachea. The gland has two lateral lobes connected by a thin tissue mass called the isthmus. Internally, each lobe comprises many small spherical structures known as thyroid follicles, surrounded by a network of blood vessels.
The follicles have a central cavity lined by simple cuboidal to squamous epithelial cells called follicular cells. These cells produce the glycoprotein...

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相关实验视频

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An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma
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使用P19胚胎癌细胞建模功能性甲状腺毛囊结构.

Fatimah Najjar1, Liming Milbauer1, Chin-Wen Wei1

  • 1Department of Pharmacology, University of Minnesota, Minneapolis, MN 55455, USA.

Cells
|November 27, 2024
PubMed
概括

研究人员使用P19胚胎癌干细胞开发了一种新的体外模型来研究甲状腺疾病. 这种无料系统有效地将干细胞分化为功能性甲状腺细胞,用于分子和遗传操纵.

科学领域:

  • 干细胞生物学 干细胞生物学
  • 内分泌学 在内分泌学.
  • 分子生物学分子生物学

背景情况:

  • 甲状腺疾病带来了挑战,因为研究甲状腺细胞发育和功能的体外模型有限.
  • 现有的模型缺乏广泛分子和遗传操纵的能力.

研究的目的:

  • 开发一种使用P19胚胎癌干细胞的新型无料体外模型.
  • 将这些干细胞分化为成熟的功能性甲状腺细胞,用于研究甲状腺疾病.
  • 为了在这个系统中实现分子和基因操纵.

主要方法:

  • 使用Activin A和甲状腺刺激激素 (TSH) 诱导P19胚胎癌干细胞.
  • 胚胎体被解离,甲状腺细胞的祖先被培养在Matrigel中.
  • 对功能性甲状腺细胞和卵泡状结构的分化和成熟进行了监测.

主要成果:

  • 该系统成功地产生了表达甲状腺蛋白的功能性甲状腺细胞,在TSH刺激时分泌了蛋白质.
  • 通过基因表达分析证实了甲状腺细胞的分化和成熟.
  • 该模型证明了分子操纵的适用性,包括siRNA引入,同时保持差异化能力.

结论:

关键词:
细胞分化的细胞分化.胚胎癌干细胞是胚胎癌的干细胞在体外模型模型中的体外模型.甲状腺细胞 甲状腺细胞甲状腺疾病 甲状腺疾病

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  • 使用P19干细胞建立了一个强大的,无料的体外甲状腺细胞模型.
  • 这种14天的系统有助于对甲状腺细胞发育和甲状腺疾病途径进行详细的分子研究.
  • 该模型支持基因操纵,为甲状腺研究提供了有价值的工具.