Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Integrated Assessment of Arterial Stiffness and Right Atrial Function in Type 2 Diabetes With Cardiovascular Autonomic Neuropathy.

Circulation journal : official journal of the Japanese Circulation Society·2026
Same author

Generation of mature epicardium derived from human-induced pluripotent stem cells via inhibition of mTOR signaling.

Nature communications·2025
Same author

Phylogenetic tree-based amino acid sequence generation for proteomics data analysis of unknown species.

Computational and structural biotechnology journal·2025
Same author

UniScore, a Unified and Universal Measure for Peptide Identification by Multiple Search Engines.

Molecular & cellular proteomics : MCP·2025
Same author

Impact of Arterial Stiffness on Cardiac Geometry.

International heart journal·2025
Same author

Human iPSC-derived nephron progenitor cells treat acute kidney injury and chronic kidney disease in mouse models.

Science translational medicine·2025

相关实验视频

Updated: Jun 6, 2025

Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation
07:15

Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

Published on: November 26, 2011

17.8K

在 ddaPASEF 和 diaPASEF 之间独特识别的的差异.

Mio Iwasaki1, Rika Nishimura1, Tatsuya Yamakawa1

  • 1Center for iPS Cell Research and Application, Kyoto University, Kyoto 606-8507, Japan.

Cells
|November 27, 2024
PubMed
概括

数据独立获取 (DIA) 和数据依赖获取 (DDA) 质谱法识别不同的,DIA识别更多独特的,特别是较短的. 这突出了蛋白质组学中特定方法的偏见.

关键词:
DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA DDA时间 时间 时间 时间数据依赖的数据采集.数据独立的获取采集.纳米LC-MS/MS/MS是什么意思- 蛋白质组的 - 蛋白质组无处不在的蛋白质组

更多相关视频

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.8K
Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
12:05

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

Published on: March 6, 2013

14.1K

相关实验视频

Last Updated: Jun 6, 2025

Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation
07:15

Amide Hydrogen/Deuterium Exchange & MALDI-TOF Mass Spectrometry Analysis of Pak2 Activation

Published on: November 26, 2011

17.8K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.8K
Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
12:05

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

Published on: March 6, 2013

14.1K

科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 质谱测量质量谱测量
  • 生物化学 生物化学

背景情况:

  • 基于质谱的蛋白质组学能够进行全面的蛋白质分析.
  • 使用机器学习的数据独立获取 (DIA) 提高了蛋白质识别效率.
  • 对DIA和数据依赖获取 (DDA) 之间的特性的比较至关重要.

研究的目的:

  • 分析通过DIA和DDA方法识别的独特的特征.
  • 根据获取方法调查基于的识别中的潜在偏差.
  • 了解翻译后修改对 DIA 和 DDA 中特性的影响.

主要方法:

  • 通过使用DDA和DIA方法识别了超过10,000种蛋白质,其中列类型相同.
  • 分析了独特的,化时间,1/K0 (疏水性) 和m/z值.
  • 对比了全细胞溶解酸与蛋白质组和无处不在的蛋白质组配置文件.

主要成果:

  • DIA发现了32%的独特,而DDA发现了19%的独特.
  • DIA优先检测较短的,特别是在化时间的前10%内.
  • 翻译后修改的显示了更宽的m/z和1/K0范围,而全方位的m/z比类低.

结论:

  • 蛋白质组学获取方法 (DIA与DDA) 在鉴定中表现出明显的偏差.
  • DIA更有效地识别较短的,并显示特定方法的化配置文件.
  • 翻译后修改丰富影响特征,需要定制的DIA参数设置.