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Updated: Jun 9, 2026

Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor
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Published on: February 1, 2015

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在NB-4细胞中增强基因传递:克服转导和选择挑战

Stefano Leto1, Sonakshi Gehlot2, Bhavwanti Sheth2

  • 1Cellular Signalling Laboratory, Department of Biomedical Sciences, University of Bologna, 40126 Bologna, Italy.

Cells
|November 27, 2024
PubMed
概括

由于毒性和促进体沉默,急性肌肉细胞白血病 (APL) 模型中的基因转导具有挑战性. 在lentiviral载体中用EF1α取代hPGK促进体,改善了NB-4细胞中的细胞活力和选择效率.

关键词:
在NB-4白血病细胞.基因转导 基因转导 基因转导病毒载体工程是病毒载体工程.

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Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
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Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.
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Transduction

Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...