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循环微RNA预测三阴性乳腺癌复发的风险

Jouni Kujala¹, Maria Tengström², Sami Heikkinen³

¹Institute of Clinical Medicine, Clinical Pathology and Forensic Medicine, University of Eastern Finland, FI-70211 Kuopio, Finland.

|November 27, 2024

概括

三重阴性乳腺癌 (TNBC) 复发风险可以使用液体活检中的循环微RNA (cmiRNA) 来评估. 三种特定的cmiRNA显示出作为预后生物标志物的潜力,用于预测TNBC复发和生存结果.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物标志物发现发现

背景情况:

三阴性乳腺癌 (TNBC) 是一种具有高复发率的攻击性亚型.
液体活检提供了一种非侵入性方法来检测癌症生物标志物.
循环微RNAs (cmiRNAs) 正在成为各种癌症的潜在预后指标.

研究的目的:

研究血清cmiRNAs作为TNBC复发的预后生物标志物的有用性.
为了比较复发性和非复发性TNBC病例之间的cmiRNA表达特征.
确定与TNBC无复发生存相关的特定cmiRNAs.

主要方法:

来自14名复发性和19名非复发性TNBC患者的血清样本被分析为cmiRNA测序.
进行了差异表达分析,以确定与复发状态相关的cmiRNAs.
用多变量生存分析来评估cmiRNAs与无复发生存的关联.
在血清和匹配的瘤cmiRNA表达特征之间进行了相关性分析.

主要成果:

在复发性和非复发性TNBC病例之间确定了10个差异表达 (DE) cmiRNA.
三种cmiRNAs (miR-21-5p,miR-16-5p,miR-26b-5p) 与没有复发的存活率有显著关联.
血清和匹配的瘤cmiRNA表达特征显示出正相关性.
德cmiRNAs与癌症相关的信号通路相关,并改善了复发风险预测模型.

结论:

复发性和非复发性TNBC表现出不同的血清cmiRNA表达特征.
特定的cmiRNAs,包括miR-21-5p,miR-16-5p和miR-26b-5p,可以作为TNBC复发风险的预后生物标志物.
血清cmiRNAs对TNBC复发的非侵入性评估和管理具有前途.

关键词:

液体活检活检液体活检测序的测序是指测序的测序.血液中的血清是血清.幸存率幸存率生存率

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...