相关实验视频
Updated: Jun 6, 2025

07:20
Author Spotlight: Imaging ATG9A, a Multi-Spanning Membrane Protein
Published on: June 16, 2023
2.0K
转录因子变形的翅膀是一种调节自的Atg8a相互作用蛋白
Marta Kołodziej1, Panagiotis Tsapras1, Alexander D Cameron1
1School of Life Sciences, University of Warwick, Coventry CV4 7AL, UK.
Cells
|November 27, 2024
概括
微管相关蛋白1轻链3 (LC3) 与转录因子相互作用 形翅膀 (Dwg) 在Drosophila. 破坏这种相互作用会增强自和抗压能力,揭示LC3在转录调节中的新型核作用.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 遗传学 遗传学 是一个
背景情况:
- 微管相关蛋白1轻链3 (LC3),也称为Atg8,对于选择性自至关重要.
- 虽然主要是细胞质的,但LC3蛋白也存在于核中,但它们的核功能和导入机制尚不清楚.
- 选择性自依赖于载荷受体上的LC3/Atg8和LIR/AIM动机之间的相互作用.
研究的目的:
- 研究Atg8家族蛋白质的核相互作用和功能.
- 为了确定与Drosophila中的Atg8相互作用的新型核蛋白.
- 阐明Atg8-变形翅膀 (Dwg) 相互作用在调节自中的作用.
主要方法:
- 酵母双杂交查以确定Drosophila中的Atg8a相互作用蛋白.
- 创建和分析具有破坏Dwg LIR动机 (Y129A/I132A) 的Drosophila突变物.
- 评估突变的自水平,氧化应激抵抗力和饥饿抵抗力.
主要成果:
- 鉴定了变形翅膀 (Dwg),一种转录因子,作为Drosophila中的新型Atg8a相互作用蛋白.
- 证实相互作用取决于Dwg.wg.中的LIR动机.
- Dwg LIR突变显示出显著增加的自和增强对氧化应激和饥饿的抵抗力.
结论:
- Atg8a和Dwg之间的相互作用在调节自中起着至关重要的作用.
- 这种相互作用对于在压力条件下维持细胞平衡是很重要的.
- 提供了对Drosophila中通过核LC3 / Atg8相互作用进行自的转录调节的新见解.
相关概念视频
Autophagy
4.2K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.2K
Regulated Protein Degradation
7.1K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.1K
Delivery Pathways to the Lysosome
6.2K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
6.2K
Autophagic Cell Death
3.4K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.4K
Canonical Wnt Signaling Pathway
8.7K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.7K
Export of Misfolded Proteins out of the ER
3.5K
After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
3.5K

