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沉默ZNF671通过激活瘤性非编码RNAs来影响头癌中的信号通路

Kendra Smith^1,2, Rufa'i Umar Zubair¹, Richard V Smith^3,4

¹Division of Biomedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, St. John's, NL A1B 3V6, Canada.

|November 27, 2024

概括

在头部和部状细胞癌 (HNSCC) 中ZNF671的表观遗传沉默与患者的生存率差相关. ZNF671的再表达抑制了癌细胞的入侵,并降低了瘤性LINC00665RNA的调节.

科学领域:

癌症表观遗传学癌症表观遗传学
分子瘤学分子瘤学
瘤抑制基因的基因

背景情况:

新的ZNF基因,包括19q13染色体上的ZNF671,在头角状细胞癌 (HNSCC) 和其他上皮瘤中经常被过甲基化.
在瘤发生过程中ZNF671的确切功能仍然在很大程度上未被描述.

研究的目的:

研究ZNF671在HNSCC中的作用.
确定HNSCC中ZNF671表观遗传沉默的功能后果.

主要方法:

在HNSCC瘤中ZNF671表达的比较与相邻的正常组织.
对ZNF671表达水平和患者生存数据的相关性分析.
在体外功能测定涉及ZNF671在口腔癌细胞系中的过度表达 (UM-SCC-1).
转录组分析以确定ZNF671.1.的下游目标.

主要成果:

与匹配的正常组织相比,ZNF671在HNSCC初级瘤中被表观遗传沉默.
较低的ZNF671表达与HNSCC患者的生存率降低显著相关.
在口腔癌细胞中过度表达ZNF671显著降低了细胞的移动性和侵入性.
ZNF671的重新表达导致瘤长非编码RNA LINC00665.5的表达显著下降.

结论:

在HNSCC中对ZNF671的表观遗传沉默可能会促进瘤发生.
由于ZNF671沉默,观察到LINC00665的上调可能有助于激活多个瘤信号通路.

关键词:

LINC0066565 在线阅读这就是ZNF671的原因.这种癌症是癌症癌症.在口腔腔的口腔.这是一种状状动物.

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