Vibrio alginolyticus通过T3SS效应器VopS重新编程CIK细胞代谢,以促进宿主细胞铁亡
Weijie Zhang1,2, Chao Huang1,2, Zhihang Chen1,2
1Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China.
Animals : an open access journal from MDPI
|November 27, 2024
概括
维布里奥·阿尔金诺利蒂克斯 (Vibrio alginolyticus) 使用VopS效应器触发宿主细胞铁,这是一种新发现的病变发生机制. 这一发现揭示了VopS是细菌感染和宿主细胞死亡必不可少的关键毒性因子.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 病原发生和发病的过程.
背景情况:
- 菌藻类病原体 (Vibrio alginolyticus) 是一种影响海洋生物和人类的重要病原体,对水产养殖造成经济损失.
- 虽然V. alginolyticus操纵宿主细胞死亡,但具体的分子机制尚未完全理解.
研究的目的:
- 调查VopS效应蛋白在V. alginolyticus.病变发生过程中的作用.
- 阐明VopS对V. alginolyticus病毒性有所贡献的分子机制.
主要方法:
- 在V. alginolyticus中构建和描述一个VopS删除突变体 (ΔvopS).
- 对细胞外蛋白酶分泌,毒性,T3SS基因表达和宿主细胞死亡 (亡和铁亡) 的分析.
- 代谢分析以确定感染期间宿主细胞代谢变化.
主要成果:
- 删除VopS显著降低了V. alginolyticus的毒性,蛋白酶分泌和T3SS基因表达.
- VopS表现出细胞毒性,通过升高的LDH和caspase-3激活诱导细胞亡.
- 通过改变上腺酸合成,耗尽谷,增加铁积累,VopS触发了宿主细胞铁亡.
结论:
- VopS效应器是V. alginolyticus的一个关键毒性因子.
- VopS诱导宿主细胞铁,代表了这种细菌的新型致病机制.
更多相关视频
08:38Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells
Published on: May 15, 2017
9.4K
04:01Author Spotlight: Tracing the Ferroptotic Signatures and Cell Death Dynamics in Medulloblastoma for Advanced Therapeutics
Published on: March 15, 2024
874
相关概念视频
01:16Necrosis
4.3K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
4.3K
01:16Viral Replication: Lysogenic Cycle
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
01:28Microbial Nutrition
1
Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...
1
01:28Gene Regulation in Microbial Communities: Quorum Sensing
3
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
3
01:27Diversity of Protists II
Alveolates are a group of organisms recognized by the presence of alveoli, which are cytoplasmic sacs located beneath the cell membrane. While their function remains uncertain, alveoli may help regulate water balance by controlling how much water enters and leaves the cell. In dinoflagellates, these structures may serve as armor plates. There are three major types of alveolates: ciliates, which move using cilia; dinoflagellates, which use flagella for movement; and apicomplexans, which are...