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在意大利牛群中发现复合艾基病毒D,意大利

Francesco Pellegrini¹, Gianvito Lanave¹, Francesca Caringella¹

¹Department of Veterinary Medicine, Università Aldo Moro di Bari, 70010 Valenzano, Italy.

Animals : an open access journal from MDPI

|November 27, 2024

概括

在牲畜中发现的RNA病毒Kobuvirus (KoVs) 在26.3%的牛中被检测到. 鉴定出了新型艾基病毒D菌株,表现出基因重组,突出显示了它们在牛病毒组中的流行程度.

科学领域:

兽医病毒学兽医病毒学
分子流行病学分子流行病学
动物健康动物健康

背景情况:

科布病毒 (KoVs) 是小的,非包裹的RNA病毒在Picornaviridae家族,已知感染人类和哺乳动物.
包括Aichivirus物种A-F在内的KoV已在各种家禽类动物中被检测到.

研究的目的:

调查牛群中科布病毒的流行率和遗传特征.
识别新的KoV菌株并了解它们在牛群中的遗传多样性.

主要方法:

反转录聚合酶链反应 (RT-PCR) 查牛便样本 (n=38).
病毒安普利康的序列分析和全基因组测序.
遗传学分析以确定遗传关系并识别重组.

主要成果:

在38个 (26.3%) 牛样本中,在10个中检测到科布病毒RNA;六个阳性动物显示肠道症状.
确定了八种菌株,称为牛中常见的艾基病毒B.
两种新型菌株,ITA/2019/572-1和ITA/2020/bovine/30-2,与艾基病毒D密切相关,与已知的菌株具有高达97.1%的核酸相同性.
全基因组分析显示,这些艾滋病毒D菌株是重组的,具有混合的D1和D2基因型.

结论:

艾奇病毒 D 科布病毒在牲畜病毒组中很常见,特别是在牛群中.
重组艾基病毒D菌株的鉴定强调了动物中KoVs的遗传多样性.
进一步了解KoV遗传多样性对于改善牲畜诊断和流行病学监测至关重要.

关键词:

一个艾奇病毒病毒.科布病毒 (Kobuvirus) 是一种病毒.这里是牛群.转基因组学是指转基因组学.再组合的复合方式.

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