关于当前可选择标记系统和新型标记器免费方法在果树基因工程中的概述
Victoria Súnico1,2, Irene Piunti1, Mamta Bhattacharjee3
1Department of Agricultural, Food, and Environmental Sciences, Marche Polytechnic University, 60131 Ancona, Italy.
International journal of molecular sciences
|November 27, 2024
概括
选择性标记基因有助于识别转基因果树. 本次审查涵盖了基因工程的选择工具和消除商业开发标记的方法.
科学领域:
- 植物生物技术 植物生物技术
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 选择性标记基因对于在转化后识别转基因细胞至关重要,特别是在低效果果树工程中.
- 传统的标记物赋予抗生素/除草剂耐药性,而像记者基因这样的视觉标记物则有助于体外鉴定.
- 在实地测试后,可选择标记物对于商业水果作物是不理想的,需要无标记物策略.
研究的目的:
- 对果树遗传工程的选择工具进行审查和评论.
- 讨论产生无标记物转基因果作物的方法.
- 强调水果物种中可选择标记基因的生物安全考虑.
主要方法:
- 关于植物遗传工程中可选择标记基因的文献综述.
- 分析传统和视觉选择工具.
- 讨论特定于位点的重组酶和编辑核酶方法,以去除标记物.
主要成果:
- 确定了传统的 (抗生素/除草剂耐药性) 和视觉的 (报告基因) 可选标记.
- 描述了使用重组酶和核酶生成无标记物转基因果树的方法.
- 强调了与可选择标记基因使用相关的生物安全方面的重要性.
结论:
- 有效的选择工具对于果树基因工程的成功至关重要.
- 无标记物策略对于转基因果作物的商业化至关重要.
- 对生物安全和有效的标记物去除的持续研究是必要的.
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