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在Pinus massoniana羊羔中识别和描述EIN3/EIL转录因子家族成员

Wenya Yu^1,2,3,4, Xingyue Ren^1,2,3,4, Jingjing Zhang^1,2,3,4

¹State Key Laboratory of Tree Genetics and Breeding, Nanjing Forestry University, Nanjing 210037, China.

International journal of molecular sciences

|November 27, 2024

概括

研究人员在马森松中发现了四种乙烯不敏感3/乙烯不敏感3类 (EIN3/EIL) 基因. 这些基因对植物生长,发育和应激反应至关重要,为松树的弹性提供了洞察力.

科学领域:

植物分子生物学植物分子生物学
文字转录学 (Transcriptomics) 是一个学科.
生物信息学是一种生物信息学.

背景情况:

转录因子调节基因表达;EIN3/EIL家族对植物生长,发育和应激反应至关重要.
尽管这棵树具有经济和生态意义,但对高精苗 (如马森松 (P. massoniana)) 中的EIN3/EIL基因的研究是有限的.

研究的目的:

为了识别和表征Mason松中的EIN3/EIL基因.
研究这些基因在应对环境压力的表达模式和潜在功能.

主要方法:

转录组测序被用来识别P. massoniana.中的假定EIN3/EIL基因.
进行了生物信息分析,以研究基因结构,保存域和分子进化.
使用定量实时PCR (qRT-PCR) 来分析不同组织和压力条件下的基因表达水平.

主要成果:

在P. massoniana中发现了四个假定的EIN3/EIL基因 (PmEILs),它们具有保存的EIN3域.
遗传学分析将PmEIL基因分为三个类别,显示了类别内保存的图案模式.
PmEIL基因在针中表达很高,在激素,透和干旱压力下表达差异,这表明它们在应激反应中的作用. PmEIL蛋白定位在核中,其中一些作为转录激活剂,一个作为抑制剂.

结论:

已识别的PmEIL基因参与马森松的生长,发育和应激反应机制.
这项研究为了解EIN3/EIL转录因子在体中的功能作用提供了基础.

关键词:

在 EIN3/EIL 中使用.松树 (Pinus massoniana) 是一种松树.表达方式表达方式表达模式压力反应应对压力的反应.转录因子的转录因子

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