相关实验视频

Updated: Jun 6, 2025

Large-Scale Multi-Omics Genome-Wide Association Studies Mo-GWAS: Guidelines for Sample Preparation and Normalization

Large-Scale Multi-Omics Genome-Wide Association Studies Mo-GWAS: Guidelines for Sample Preparation and Normalization

Published on: July 27, 2021

通过全基因组关联模型在珍珠中发现了与抗爆基因相关的新型SNP

Swati Singh¹, Ganesan Prakash², Sandeep Nanjundappa¹

¹Genomics and Molecular Breeding Lab, Global Centre of Excellence on Millets (Shree Anna), ICAR-Indian Institute of Millets Research, Hyderabad 500030, India.

International journal of molecular sciences

|November 27, 2024

概括

使用全基因组关联研究 (GWAS) 调查了珍珠小米弹性耐药性. 这项研究确定了与耐药性相关的关键基因和基因组区域,为改进作物育种策略铺平了道路.

科学领域:

植物病理学植物病理学
遗传学是一个遗传学.
农业学是一种农业学.

背景情况:

由 *Pyricularia grisea* 引起的叶片爆发,显著降低了珍珠小米 (*Pennisetum glaucum*) 的产量,特别是在受雨影响的地区.
了解防爆耐药性的遗传基础对于开发弹性珍珠小米品种至关重要.

研究的目的:

通过全基因组关联研究 (GWAS) 识别与珍珠小米的爆裂耐药性相关的遗传位置.
探索涉及赋予抗 *Pyricularia grisea * * 的基因和途径.

主要方法:

一项全基因组关联研究 (GWAS) 在281个多样化的珍珠小米杂交系进行.
收集了针对三个 *P. grisea* 隔离物抗爆的表型数据.
贝叶斯信息和链接不平衡代嵌套关键路 (BLINK) 和多位点混合模型 (MLMM) 用于识别显著的单核酸多态 (SNP).

主要成果:

观察到爆发性耐药性的显著遗传变异性,其中16.7%的杂交动物表现出高耐药性.
确定了68个与抗爆相关的显著SNP,其中染色体1,2和6上的热点.
参与免疫反应,耐压,信号转导和转录调节的基因被发现与耐药性有关.

结论:

这项研究提供了宝贵的见解,珍珠小米的抗爆耐药性遗传结构.
识别出与耐药性相关的基因和基因组区域可以支持标记器辅助育种计划和基因编辑策略,以提高作物保护.

关键词:

在GWAS中,GWAS就是GWAS.生物压力是生物压力.爆炸爆炸爆炸的爆炸.疾病耐药性疾病耐药性珍珠小米小米珍珠小米抗性基因是抗性的基因.

更多相关视频

A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information

A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information

Published on: July 1, 2020

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Published on: August 21, 2016

相关概念视频

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...