联储批量战略实现了从预处理的玉米炉和玉米中生产高度发酵糖溶液和纤维素乙醇
Jiamin Huang1, Xuezhi Li1, Jian Zhao1
1State Key Laboratory of Microbial Technology, Shandong University, No. 72, Binhai Road, Qingdao 266237, China.
International journal of molecular sciences
|November 27, 2024
概括
通过优化料批次酶化水解,有效地将纤维素生物质转化为高度可发酵糖和乙醇. 这种方法绕过了糖度,使得直接的大规模发酵成为可持续生物燃料生产的途径.
科学领域:
- 生物技术是生物技术.
- 可再生能源可再生能源是可再生能源.
- 生物质转换生物质转换
背景情况:
- 纤维素生物质为生产液体燃料和化学品提供了化石燃料的可持续替代品.
- 有效的转化需要高度的可发酵糖溶液进行直接的大规模发酵.
研究的目的:
- 研究料批量策略和酶性水解条件,以有效地进行预处理的玉米和玉米残留物的高固体加载水解.
- 为了达到高度的可发酵糖,适合直接发酵.
- 通过同时的糖化和共发酵来生产纤维素乙醇.
主要方法:
- 玉米炉 (ACSE-CS) 和玉米残留物 (CCR) 的酸催化蒸汽爆炸预处理.
- 在高固体负载下优化Feed-batch酶性水解.
- 同时糖化和共发酵 (SSCF) 用于乙醇生产.
主要成果:
- 在高固体载荷下获得161.2g/L (ACSE-CS) 和205g/L (CCR) 的发酵糖溶液 (分别为30%和27%).
- 在72小时内实现高葡萄糖转化率:ACSE-CS为80.9%,CCR为87.6%.
- 在SSCF的144小时后,达到46.1g/L (ACSE-CS) 和72.8g/L (CCR) 的乙醇度.
结论:
- 优化料批次的酶化水解使得在没有缩步骤的情况下,可以从木纤维素生物质中生产高度糖溶液.
- 开发的方法适合在大型发酵过程中直接应用.
- 这项研究为制造纤维素乙醇的纤维素生物质生物质创建"糖平台"提供了有价值的参考.
相关概念视频
01:22Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
01:14Batch vs Continuous Culture
Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...
01:23Fed-Batch Culture
Fed-batch culture is a widely used bioprocessing strategy combining aspects of batch culture with controlled substrate feeding to optimize cell growth and product formation. In this semi-closed system, nutrients are strategically added during fermentation, while the accumulated products and biomass remain within the bioreactor until the end of the operation. This controlled addition of substrates allows for better management of growth kinetics, nutrient limitation, and metabolite...
01:27Production of Alcohol
Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...
01:25Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
01:25Biofuels
The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...