化的丝虫Bombyx mori的T2T基因组
Wan-Shun Li1, Ying-Dan Xiao1, Jian-Qiu Liu1
1Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing 400715, China.
International journal of molecular sciences
|November 27, 2024
概括
研究人员介绍了第一个端粒到端粒 (T2T) 基因组组合Bombyx mori,一个关键的昆虫模型. 这种高质量的基因组提高了对丝生产的理解,并为未来的昆虫基因组项目提供了宝贵的参考.
科学领域:
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
- 昆虫生物学 昆虫生物学
背景情况:
- 基因组序列是物种生物学的基础.
- 高通量测序和生物信息学已经推进了基因组组装.
- 博姆比克斯·莫里 (Bombyx mori) 是生物研究中一个重要的模型生物.
研究的目的:
- 为了呈现第一个端粒到端粒 (T2T) 基因组组装Bombyx mori.
- 为B. mori.提供高质量,完整的基因组序列.
- 为未来的T2T基因组组合提供见解和策略.
主要方法:
- 集成HiFi,超长时间ONT,NGS和Hi-C测序数据.
- 综合的基因组组装和注释过程.
- 使用BUSCO进行基因完整性和Merqury进行共识质量 (QV) 的质量评估.
主要成果:
- 组装的T2T基因组跨越28个染色体的450,267,439个基因对.
- 标注鉴定了18,253个编码蛋白质的基因,其中99.1%的单拷贝基因完整性得到保护.
- 获得了B. mori的最高重复序列比例 (60.77%),具有卓越的端粒和并列区域的代表性.
结论:
- 这项研究为Bombyx mori.提供了第一个完整的T2T基因组组合.
- 与以前的B. mori基因组相比,组件提供了更高的质量和完整性.
- 分享的方法和经验将指导未来的其他物种T2T基因组项目.
相关概念视频
Transgenic Organisms
30.9K
Overview
30.9K
DNA-only Transposons
14.4K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
14.4K
Exon Recombination
3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.6K
Genomic DNA in Eukaryotes
46.7K
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
46.7K
LTR Retrotransposons
17.3K
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
17.3K


