产生神经递质的人类皮肤同类的多样性
Samane Rahmdel1, Moushumi Purkayastha1, Mulugeta Nega1
1Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine Tübingen (IMIT), University of Tübingen, 72076 Tübingen, Germany.
International journal of molecular sciences
|November 27, 2024
概括
皮肤细菌,主要是葡萄球菌,可以产生像多巴胺和血清素这样的神经递质. 两个酶,Sada和TDC,解释了不同的生产概况,但它们的宿主效应需要进一步研究.
科学领域:
- 微生物学 微生物学
- 神经科学是一个神经科学.
- 生物化学 生化学
背景情况:
- 人类微生物群,包括肠道和皮肤细菌,可以产生神经递质 (NT),如多巴胺和血清素.
- 这些NTs可以通过影响经典信号通路或与微量胺相关受体 (TAARs) 相互作用来影响宿主生理学.
- 与肠道微生物群相比,皮肤微生物群对NT的产生存在有限的研究.
研究的目的:
- 研究皮肤细菌分离物产生神经递质的能力.
- 为了确定产生的特定神经递质和涉及的细菌属.
- 阐明皮肤细菌中神经递质产生背后的酶机制.
主要方法:
- 1909年皮肤细菌分离物的查 (主要是葡萄球菌,细菌,Corynebacterium) 用于NT生产.
- 基于基质特异性的NT生产概况的表征.
- 鉴定和对参与NT合成的SADA和TDC酶进行比较分析.
- 在大肠杆菌中sadA和tdc基因的异质表达以确认酶功能.
主要成果:
- 只有6.7%的皮肤分离物产生了NTs,仅限于Staphylococcus属.
- 观察到两个不同的NT生产配置文件:一个生产胺 (TRY) 和乙胺 (PEA),另一个生产胺 (TYM) 和多巴胺 (Dopa).
- 萨达酶与TRY/PEA生产有关,而TDC酶与TYM/Dopa生产有关,其大小,结构和基因组定位不同.
结论:
- 皮肤微生物群,特别是葡萄球菌种类,具有合成各种神经递质的能力.
- 酶SadaA和TDC在确定这些细菌产生的NTs的特定光谱方面发挥着不同的作用.
- 需要进一步的研究来了解皮肤微生物群衍生的NTs对人类神经受体信号传递的生理影响.
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