两种脱细胞化方案对人类尿道的机械行为和结构性质的影响
Marcela Kuniakova1, Zuzana Varchulova Novakova1, Daniel Haspinger2
1Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University in Bratislava, Sasinkova 4, 811 08 Bratislava, Slovakia.
International journal of molecular sciences
|November 27, 2024
概括
两种脱细胞化方法 (酶-洗剂和洗剂-洗剂) 有效地保护了人尿道外细胞矩阵结构和生物机械特性,用于组织工程和生物银行. 这两种方法都没有显示出细胞毒性,支持它们的潜在临床应用.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
背景情况:
- 脱细胞化对于为组织再生创造生物相容的支架至关重要.
- 人类尿道组织支架是治疗尿道狭窄和其他疾病所需的.
- 评估不同的脱细胞化协议对于优化支架特性至关重要.
研究的目的:
- 为了比较酶-洗剂 (ED) 和洗剂-洗剂 (DD) 脱细胞化方案对人类尿道组织的影响.
- 评估脱细胞化尿道支架的结构完整性和生物力学特性.
- 评估这些支架的生物相容性和用于组织工程和生物银行的潜力.
主要方法:
- 人类尿道组织使用ED (n=7) 和DD (n=11) 协议进行脱细胞化.
- 组织学,超结构和免疫组织化学分析评估了细胞外基质 (ECM) 保存.
- 进行了生物力学测试 (考希应力,失效时伸展,弹性模量) 和细胞毒性测试.
主要成果:
- 两个ED和DD协议都有效地去除了细胞组件,同时保留了ECM结构,包括原和弹性纤维.
- 脱细胞化的组织显示完整的IV原和纤维菌素.
- 与原生组织相比,DD组织表现出明显较低的Cauchy应力;两个脱细胞化组都减少了失效时的拉伸和弹性模量. 没有观察到细胞毒性.
结论:
- 无论是ED还是DD脱细胞化协议,都保留了人类尿道ECM结构和生物力学特性.
- 这些脱细胞化组织适合在组织工程移植和生物银行中潜在使用.
- 需要进一步优化,以增强支架再细胞化,并确保临床疗效.
相关概念视频
Physiology of Urine Formation
Urine formation is an essential function of the human body. It plays a critical role in maintaining homeostasis by regulating the volume and composition of body fluids. The kidneys, the primary organs involved in this process, filter blood to remove waste products and excess substances, ultimately producing urine.
Glomerular Filtration
The first stage in urine formation is glomerular filtration. Each kidney contains approximately 1 million nephrons, the functional units of filtration, with a...
Glomerular Filtration
The first stage in urine formation is glomerular filtration. Each kidney contains approximately 1 million nephrons, the functional units of filtration, with a...
Urinary Tract Infection II: Pathophysiology
The pathophysiology of urinary tract infections (UTIs) encompasses several progressive stages, beginning with bacterial colonization and culminating in potential systemic complications if untreated. UTIs are primarily initiated by bacteria, such as Escherichia coli, which often originate from the gastrointestinal tract and migrate to the urinary system through the periurethral area. This migration can occur via several routes, including improper hygiene practices, sexual activity, or...
Microbiota of the Urogenital Tract
The human urogenital system, once thought to be sterile in healthy individuals, is now recognized as a complex microbial habitat. Advancements in molecular sequencing techniques have revealed that even in healthy adults, the kidneys and bladder harbor microbial populations similar to those found in the distal urethra, albeit in much lower abundance. These resident microorganisms, while generally innocuous, can become opportunistic pathogens under conditions that alter the urogenital...
Cellular Adaptation IV: Dysplasia and Metaplasia
DysplasiaDysplasia refers to abnormal changes in the size, shape, and organization of mature cells, characterized by pleomorphism, nuclear abnormalities, and increased mitotic activity. It commonly affects epithelial tissues, including the cervix, gastrointestinal tract, respiratory mucosa, and endometrium. Although it may occur alongside hyperplasia, dysplasia is not a true adaptive response but a preneoplastic change with potential to progress to cancer.When confined above the basement...


