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Updated: Jun 6, 2025

CRISPR Guide RNA Cloning for Mammalian Systems
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Published on: October 2, 2018

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可光切割的指南crRNAs用于可光控制的CRISPR/Cas9系统

Lubov Sakovina1,2, Ivan Vokhtantsev1,2, Elizaveta Akhmetova1,2

  • 1Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, Russia.

International journal of molecular sciences
|November 27, 2024
PubMed
概括

研究人员开发了可光分离的指导CRISPRRNA (crRNA),可以用UVA光灭. 这种方法通过减少非目标效应来提高CRISPR/Cas9基因组编辑精度.

关键词:
2′-修饰的crRNARNA被使用.非目标效应的非目标效应可光分解的crRNARNA摄影调节的CRISPR/Cas9系统

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