相关实验视频

Updated: Jun 6, 2025

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

Published on: February 3, 2022

作为复制DNA修复的控制器的POLD3

Nabilah Alli¹, Anna Lou-Hing¹, Edward L Bolt¹

¹School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK.

International journal of molecular sciences

|November 27, 2024

概括

尽管它具有突变性DNA合成,但POLD3亚单元对真核DNA修复和细胞存活至关重要. 了解POLD3的结构和功能是DNA修复机制和人类健康的关键.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
生物化学生物化学

背景情况:

DNA修复途径依赖于由酶如真核生物B家族DNA聚合酶进行的DNA合成.
基因组聚合酶delta (Polδ) 和zeta (Polζ) 对于通过同源重组或单链回火来修复DNA链断裂至关重要.
虽然Polδ和Polζ介导的合成是致变的,但在存在DNA断裂时,它对细胞生存至关重要.

研究的目的:

阐明POLD3亚单元的发现,结构和细胞功能.
探索POLD3.3尚未探索的结构方面和新的生物化学数据.
了解POLD3在真核DNA修复,突变发生及其对人类健康的影响中的关键作用.

主要方法:

关于POLD3发现的文献综述.
关于POLD的结构调查3.
在细胞DNA修复中对POLD3功能的生物化学分析.

主要成果:

通过重组,调节聚合酶活性和与修复蛋白相互作用,POLD3是DNA修复的核心.
新的结构和生物化学数据为POLD3的功能提供了洞察力.
这项研究强调了POLD3在DNA修复和突变发生过程中的关键作用.

结论:

POLD3是真核细胞DNA修复中的一个关键蛋白质,影响细胞生存和突变发生.
进一步对POLD3的结构和生化研究对于全面了解其作用至关重要.
了解POLD3的功能对人类健康和疾病有影响.

关键词:

修复DNA的修复DNA的修复复制DNA复制DNA复制DNA复制波兰3 波兰3 波兰人波兰人波兰人波兰人螺旋酸的使用方法基因组基因组基因组

更多相关视频

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

相关概念视频

DNA Damage can Stall the Cell Cycle

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

Translesion DNA Polymerases

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...

Restarting Stalled Replication Forks

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...

Overview of DNA Repair

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...

Negative Regulator Molecules

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.