循环时钟PER2基因过度表达对膜上皮细胞动力学和VFA运输蛋白表达的影响
Rahmat Ali1, Yongkang Zhen1, Xi Zanna1
1Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
International journal of molecular sciences
|November 27, 2024
概括
过度表达羊细胞中的PER2基因会抑制细胞生长并增加细胞亡. 这表明PER2可能通过影响短链脂肪酸转运体来调节营养吸收.
科学领域:
- 分子生物学分子生物学
- 动物科学动物科学
- 动物生理学 动物生理学
背景情况:
- 昼夜基因PER2影响非反动物细胞中的细胞增殖和脂质代谢.
- 了解昼夜基因在反动物的营养同化中的作用,对于畜牧业的生产力至关重要.
研究的目的:
- 为了研究PER2基因过度表达对山羊皮上皮细胞的影响.
- 评估PER2对昼夜基因表达,细胞增殖,细胞亡和VFA载体mRNA水平的影响.
主要方法:
- 羊皮上皮细胞在体外培养,并用pcDNA3.1-PER2等离子体转染,以过度表达.
- 使用定量PCR测量PER2,细胞循环调节剂,炎症标志物和VFA载体的mRNA水平.
- 细胞亡率和细胞增殖在传染后24小时和48小时被评估.
主要成果:
- 过度表达PER2显著增加了亡率,并减少了皮表皮细胞中的细胞增殖.
- PER2的升高调节了细胞周期基因 (CCND1,WEE1,p21,p16,CDK4) 的表达,并增加了TNF-α.
- 过度表达PER2改变了VFA载体的mRNA水平,降低AE2,NHE1,MCT1,MCT4的调节,并提高PAT1,VH+ ATPase的调节.
结论:
- 过度表达PER2基因会损害细胞增殖,并增强山羊皮上皮细胞的亡.
- PER2 影响 VFA 载体表达,这表明它在调节乳头中的 VFA 吸收方面发挥了作用.
- 需要进一步的研究来阐明PER2在反动物的营养同化中的精确调节机制.
关键词:
在PER2基因基因中,昼夜节律 昼夜节律膜上皮质 (rumen epithelium) 是一种表皮的组成部分.昼夜时间表 (Circadian Clock) 是一个生理时钟.挥发性脂肪酸载体蛋白质是挥发性的脂肪酸载体蛋白质.更多相关视频
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