在分化神经细胞中,氧化应激相关的circRNA及其ceRNA网络的改变
Ebrahim Mahmoudi1,2, Behnaz Khavari1,2, Murray J Cairns1,2
1School of Biomedical Sciences and Pharmacy, The University of Newcastle, Newcastle, NSW 2308, Australia.
International journal of molecular sciences
|November 27, 2024
概括
氧化应激会影响神经发育. 这项研究揭示了循环RNAs (circRNAs) 如何形成一个调节网络,为与环境暴露相关的神经发育障碍提供了洞察力.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 遗传学 遗传学是一种遗传学.
背景情况:
- 环境暴露会导致氧化应激,可能导致神经发育障碍.
- 了解分子机制,特别是监管网络,对于解决这些影响至关重要.
研究的目的:
- 在细胞氧化应激模型中分析由循环RNAs (circRNAs) 介导的竞争性内源RNA (ceRNA) 网络.
- 在氧化应激条件下的神经分化过程中研究circRNAs的作用.
主要方法:
- 在SH-SY5Y细胞模型上利用RNA测序和in silico分析.
- 构建了一个circRNA-microRNA-messengerRNA (miRNA-mRNA) 网络,以确定关键的调控相互作用.
主要成果:
- 确定了146个差异表达的circRNAs (93个上调,53个下调).
- 发现了15个作为网络枢纽的circRNAs,其中的目标基因参与压力和神经元通路,如VEGF信号传递,轴突引导,FGFR信号传递和RAF/MAP激酶级联.
- 观察到circRNAs和mRNAs之间显著的共同表达,可能与miRNAs相互作用.
结论:
- 循环RNA介导的ceRNA网络在神经元分化过程中的氧化应激中起着重要作用.
- 这些发现为与氧化压力相关的神经发育障碍提供了潜在的分子解释.
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