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多组学方法揭示了关键的mRNA-miRNA-lncRNA网络,调节了山羊卵巢中的多个出生特征

Weibing Lv^1,2,3,4, Ren An^1,2,3,4, Xinmiao Li^1,2,3,4

¹College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China.

International journal of molecular sciences

|November 27, 2024

概括

研究人员探索了山羊生殖表现的分子基础,确定了影响生育和适应能力的关键基因网络. 这项研究为更适合西藏高原环境的养殖山羊提供了基础.

科学领域:

动物遗传学和动物繁殖
生殖生物学生殖生物学
分子机制的分子机制

背景情况:

在西藏高原上养山羊需要提高生育能力和适应能力.
卵巢功能对生殖性能至关重要,由复杂的基因网络调节.
羊卵巢中mRNA-miRNA-lncRNA网络的分子机制在很大程度上是未知的.

研究的目的:

调查山羊卵巢中的组织学差异和差异性基因表达.
确定影响生殖表现的关键mRNA-miRNA-lncRNA调节网络.
了解这些网络在高原适应能力中的作用.

主要方法:

关州黑山羊 (CBG) 和西藏山羊 (TG) 卵巢的组织形态学分析.
RNA测序 (RNA-Seq) 和小RNA-Seq用于识别差异表达基因 (DEG).
构建和分析mRNA-miRNA-lncRNA相互作用网络.

主要成果:

与TG相比,CBG卵巢的脑髓比例显著降低.
确定了1218个DE mRNA,100个DE miRNA和326个DE lncRNA.
发现了五个关键的mRNA-miRNA-lncRNA网络,调节卵巢功能,生殖细胞和卵细胞发育,影响免疫和代谢能力,适应高原.

结论:

鉴定出新的mRNA-miRNA-lncRNA网络,对于调节山羊卵巢功能和生殖能力至关重要.
这些发现为西藏高原山羊的分子育种策略提供了见解.
为开发具有增强生殖能力和高原适应能力的山羊品种提供了理论基础.

关键词:

楚安忠黑山羊 (CBG) 是一个黑山羊.西藏山羊 (TG) 是一种西藏山羊.ceRNA 网络是什么意思多重出生特征的多重出生特征卵巢卵巢是一个卵巢.

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