在小麦遗传种群中测量粉耐药性的定量特征位置映射
Zhiyong Zhao1, Yuliang Qiu1, Menglin Cao1
1Institute of Cotton Research, Shanxi Agricultural University, Yuncheng 044000, China.
Genes
|November 27, 2024
概括
研究人员确定了小麦中粉状菌耐药性的三个稳定的定量特征基点 (QTL),包括6A染色体上的新型QTL. 这些发现有助于通过标记器辅助选择开发抗病小麦品种.
科学领域:
- 植物遗传学 植物遗传学
- 农业科学 农业科学
- 分子生物学分子生物学
背景情况:
- 粉状菌显著影响小麦产量和质量.
- 识别耐药基因对于小麦的标记器辅助育种至关重要.
- 之前的研究重点是了解这种流行疾病的遗传抵抗力.
研究的目的:
- 描述和精确地绘制与小麦粉耐药性相关的定量特征位点 (QTL).
- 为了确定新的遗传基因位点赋予耐药性粉状菌.
- 在育种计划中开发用于标记器辅助选择 (MAS) 的分子标记物.
主要方法:
- 开发了一种高密度的35K DArT遗传链接地图,来自双倍单 haploid (DH) 群体.
- 交叉了一个高度耐药的小麦线 (金迈33号) 与一个易受影响的线 (扬农19号).
- 采用复合间隔图形和多重间隔映射来识别和映射QTLs.
主要成果:
- 在染色体1B,2B和6A上确定了三种粉状菌耐药性的稳定QTL.
- QTL解释了表型变异 (PVE) 从4.98%到13.25%.
- 在染色体6A上发现了一种新的QTL,Qpm.sxn-6A,来自男性父母的抗性.
结论:
- 在整个环境中,Qpm.sxn-1B和Qpm.sxn-2B都被一致识别出来,金迈33的协同效应.
- Qpm.sxn-1B是一个主要的稳定QTL,而Qpm.sxn-2B位于Pm51.1.附近.
- 为MAS选择了五种分子标记物来跟踪Qpm.sxn-1B和Qpm.sxn-2B,以促进粉状菌耐药性的繁殖.
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