用CRE殖民的患者的积极监测:基于组合分子/培养协议的单中心研究
Beatrice Silvia Orena1, Maria Francesca Liporace1, Antonio Teri1
1Clinical Microbiology and Virology Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy.
Antibiotics (Basel, Switzerland)
|November 27, 2024
概括
分子查对于在入院时检测耐卡巴的Enterobacteriaceae (CRE) 载体至关重要. 这种方法为传统的培养查提供了一个重要的补充,用于在重症监护机构有效控制感染.
科学领域:
- 临床微生物学 临床微生物学
- 传染性疾病 传染性疾病
- 分子诊断学 分子诊断
背景情况:
- 耐卡巴尼姆的Enterobacteriaceae (CRE) 构成了严重的全球健康威胁.
- 积极监测对于早期检测和控制CRE至关重要.
- 住院患者,特别是高危病房的患者,容易受到CRE殖民的感染.
研究的目的:
- 分析通过多重RT-PCR检测到的CRE在直肠拭子中的流行病学.
- 为了比较分子查与传统培养方法对CRE检测的有效性.
- 评估分子方法在CRE积极监测中的作用.
主要方法:
- 预期从入院患者中收集2861个直肠拭子.
- 使用多重RT-PCR (AllplexTM Entero-DR Assay) 的分子查用于卡巴酶基因 (KPC,NDM,VIM,IMP,OXA-48) 的分子查.
- 在MacConkey和CHROMID® CARBA板上进行培养测试,通过分子检测测试样品呈阳性或无效.
主要成果:
- 在2861人中,有118人 (约. 4%) 的样本通过分子检测测出至少一个碳烯酶基因.
- 检测到的KPC,NDM和VIM是最常见的碳烯酶基因.
- 在75.4%的阳性样本中,培养证实了碳烯酶,在方法之间有25%的不一致率,在VIM.上升到60%.
结论:
- 对于CRE载体的分子查对于重症监护中的积极监测协议至关重要.
- 分子方法可以快速识别碳烯酶基因,补充基于培养的检测.
- 通过使用分子诊断技术及时识别CRE载体,可以实施改进的感染控制措施.
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