关于一些微生物多基菌的抗菌性质的研究来源于Monascus菌株
Daniela Albisoru1, Nicoleta Radu1,2, Lucia Camelia Pirvu3
1Faculty of Biotechnologies, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Boulevard, District 1, 011464 Bucharest, Romania.
Antibiotics (Basel, Switzerland)
|November 27, 2024
概括
这项研究验证了Monascus polyketides对细菌和真菌的抗菌潜力. 虽然有希望,但它们的毒性表明局部应用比系统使用更安全.
科学领域:
- 微生物学和天然产品化学
- 抗微生物耐药性研究研究
背景情况:
- 抗微生物耐药性是全球卫生挑战,推动了对新型治疗分子的研究.
- 众所周知,Monascus polyketides具有潜在的抗菌活性,但需要进一步调查.
- 有限的研究存在于来自Monascus菌株的原生生物产品的抗菌特性.
研究的目的:
- 评估含有Monascus polyketides的六种原始生物制品的抗菌性质.
- 研究这些化合物对各种细菌和真菌病原体的疗效.
- 评估这些天然产品作为传统抗微生物药物的替代品的潜力.
主要方法:
- 使用三种Monascus菌株进行Monascus polyketides固态生物合成.
- 使用PyMOL v3.0.4.4的抗微生物活性在基预测.
- 电子喷雾电离质谱仪 (ESI-MS) 用于化合物识别.
- 在体外抗微生物测定,包括Kirby-Bauer扩散方法.
主要成果:
- 在分析中确定了具有高抗微生物亲和力的Monascorubramine和黄色多基基 (Ankaflavin,Monascin).
- 药物动力学预测表明吸收良好,并穿透血脑屏障,但也有潜在的药物代谢酶抑制和毒性.
- 在体外研究证实了对黄金葡萄球菌 (Staphylococcus aureus),白菌 (Candida albicans) 和真菌皮肤菌的显著抗菌活性.
- 在实验室中得到验证的结果对埃舍里希亚大肠杆菌对Pseudomonas aeruginosa,Salmonella enterica和Serratia marcescens进行了in silico预测.
结论:
- 莫纳斯克多基虫 (Monascus polyketides) 对一系列病原体表现出经过验证的抗微生物特性,通过in silico和in vitro方法证实了这一点.
- 这些已识别的化合物显示出抗微生物应用的潜力.
- 由于观察到的毒性,建议局部配方用于更安全地使用这些生物制品.
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