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在Myzus persicae携带棕黄病毒中的miRNAs的分子表征

Meng-Jun He¹, Yun Wang¹, Mei Zhao¹

¹Ministry of Agriculture and Rural Affairs Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China.

|November 27, 2024

概括

棕黄病毒 (BrYV) 在绿色桃花 (Myzus persicae) 中显著改变了微RNA (miRNA) 的表达. 这些miRNA配置文件的变化突出了潜在的分子机制,这些机制是虫病毒传播的基础.

科学领域:

分子生物学分子生物学
病毒学病毒学
昆虫学昆虫学是一门学科.

背景情况:

微RNAs (miRNAs) 是基因表达在转录后水平的关键调节者.
在Myzus persicae和Brassica yellows病毒 (BrYV) 之间的相互作用中,miRNAs的作用尚不清楚.
在不同的宿主植物和病毒条件下研究虫中的miRNA配置文件对于理解植物-病原体相互作用至关重要.

研究的目的:

为了表征Myzus persicae中微RNA (miRNA) 资料,以应对布拉西卡黄色病毒 (BrYV) 感染.
在不同的宿主植物 (卜与阿拉比多普西斯塔利亚纳) 和病毒 (无BrYV与携带BrYV) 条件下,在虫中识别差异表达的miRNA.
阐明 BrYV 感染的虫中差异表达的 miRNA 准的潜在分子通路.

主要方法:

在Myzus persicae种群上进行了miRNA分析,两种不同的治疗方法:在卜植物上生长和在Arabidopsis thaliana上生长.
对于每种治疗方法,在非病毒性和病毒性状态下分析了虫.
进行了差异表达分析和KEGG丰富分析,以确定显著的miRNA变化及其向途径.

主要成果:

在所有实验组中发现了大量已知和新型成熟的miRNA.
在携带BrYV的虫中观察到miRNA的显著差异表达,特别是在Arabidopsis thaliana治疗下,有八个上调和四个下调miRNA.
凯格丰富分析表明,差异表达的miRNAs的基因主要参与诸如Peroxisome,神经活性联体受体相互作用和通过cytochrome P450.0.的异生菌代谢等途径.

结论:

BrYV感染的程度影响了Myzus persicae中的miRNA表达模式.
作为对BrYV感染的反应,差异表达的miRNA向关键的生物通路,这表明它们参与了虫病毒相互作用.
这些发现为虫传播BrYV的分子机制提供了宝贵的见解,并为未来的研究打开了道路.

关键词:

生物信息学是一种生物信息学.布拉西卡黄色病毒病毒绿色桃花虫绿色桃花虫微RNAs 是一个微型RNA.波莱罗病毒 (polerovirus) 是一种病毒.在sRNA测序过程中,

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