在海底深层沉积物中,在高水静压下,真菌的甲生产
Mengshi Zhao1, Dongxu Li1, Jie Liu2
1State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210023, China.
Microorganisms
|November 27, 2024
概括
深海真菌在高水立压下 (HHP) 增强甲产量. 这项研究揭示了压力诱导的氧化应激增强真菌无氧甲合成,影响全球碳循环.
科学领域:
- 微生物学 微生物学
- 地质化学 地质化学
- 生物地质化学生物地质化学
背景情况:
- 深层地下沉积物中的真菌可以无氧地产生甲.
- 这些真菌在高水静压 (HHP) 下产生的甲还不清楚.
研究的目的:
- 研究HHP对深海真菌*Schizophyllum commune*无氧甲产生的影响.
- 阐明压力诱导的甲生产变化背后的分子机制.
主要方法:
- 在模拟的现场条件下培养*S. commune* (30°C,35 MPa HHP和0.1 MPa大气压) 5天.
- 分析生物活性,生物质和转录组学以评估甲生产和基因表达.
- 测量细胞内活性氧物种 (ROS) 水平并进行比较治疗.
主要成果:
- *S. commune*幸存下来,与大气压相比,在HHP下,甲产量在大气压下大约增加了2.5倍.
- 在HHP下,参与甲合成的基因 (mct1,dh3) 的上调分别为7.9倍和12.5倍.
- 甲产量的增加与压力诱导的氧化应激有关,其证据是ROS水平升高.
结论:
- 高水立压会增加真菌无氧甲的产量.
- 氧化应激在调解真菌甲合成压力效应方面发挥着关键作用.
- 在深层地下环境中,真菌可能对全球甲流量作出重大贡献.
关键词:
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