使用生物信息学分析和实验验证的方法,寻找抗动脉样硬化联合管理的子-贝西尔的枢纽基因
Sendaer Hailati1,2,3,4, Meng-Yuan Han1,2,3,4, Dilihuma Dilimulati1,2,3,4
1Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China.
Pharmaceuticals (Basel, Switzerland)
|November 27, 2024
概括
这项研究确定了四个关键基因 (COL1A1,COL3A1,BGLAP,NOX4) 参与动脉样硬化 (AS) 治疗. (COM) 和玄米 (OB) 的联合使用有效地降低了AS指标,并验证了这些枢纽基因.
科学领域:
- 药理学 药理学 是一个学科.
- 生物信息学是一种生物信息学.
- 心血管研究研究心血管研究
背景情况:
- 动脉样硬化 (AS) 是全球疾病和死亡的主要原因.
- 维吾尔族传统药物, (COM) 和大 (OB),已被公认为对心血管有益.
- 了解AS中COM-OB联合使用的分子机制至关重要.
研究的目的:
- 为了识别和验证与 (COM) 和玄米 (OB) 联合治疗动脉样硬化 (AS) 相关的枢纽基因.
- 为了研究COM-OB同时对AS的治疗效果,在体外和体外模型中.
主要方法:
- 使用网络药理学和生物信息学分析来确定潜在的药物和疾病点.
- 试验室 (HUVEC) 和体内 (斑马鱼) 模型被用于评估COM-OB.OB的治疗疗效.
- 进行了西斑分析,以验证已识别的枢纽基因的表达.
主要成果:
- 网络分析得出3302种药物标,1963种疾病标和1630种差异表达基因 (DEGs).
- 四个枢纽基因 (COL1A1,COL3A1,BGLAP,NOX4) 通过分子对接和动态被确定并验证.
- 同时使用COM-OB显著降低了HUVEC细胞中的脂质沉积和斑马鱼中的脂肪积累.
结论:
- 四个枢纽基因,COL1A1,COL3A1,BGLAP和NOX4,被确定并验证为AS治疗的关键标.
- 这些发现为在治疗动脉样硬化时使用 (COM) 和玄米 (OB) 的同时使用提供了科学依据.
- 这项研究阐明了COM-OB在心血管疾病中的治疗潜力的基础分子机制.
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