简单批量和集中批量生物反应器培养复合BCG的比较
Sarah Mendes1,2, Maria C P Gonçalves1, Vitoria A P Aiex1
1Instituto Butantan, São Paulo 05503-900, Brazil.
Pharmaceutics
|November 27, 2024
概括
使用L-glutamic acid进行重组BCG-pertussis的料批培养,在冷干燥后改善了细胞活力,减少了整体培养时间. 这种方法增强了双重保护候选疫苗的生产.
科学领域:
- 微生物学和生物技术
- 疫苗开发 疫苗开发
- 发酵技术的发酵技术
背景情况:
- 结核病 (TB) 仍然是一个主要的全球健康威胁,现有的Bacillus Calmette-Guérin (BCG) 疫苗需要生产现代化.
- 复合BCG-百日咳 (rBCG-百日咳) 菌株提供了潜在的双重防治结核病和百日咳,并提高了膀癌的疗效.
- 研究了水下培养技术,以提高rBCG-pertussis的生产力和标准化.
研究的目的:
- 为了研究使用L-胺酸生产rBCG-百日咳的料批培养策略.
- 评估pH-stat控制对rBCG-pertussis生长和生存能力的影响.
- 评估食批量培养在冷干燥后细胞恢复的有效性.
主要方法:
- 采用了具有pH-stat控制的食批量培养策略,将L-谷氨酸入1L生物反应器.
- 对于食批量种植,测试了三种不同的pH值,用于比较使用了简单的批量培养.
- 在培养期间采集的样本的冷干燥前后测量了活细胞度.
主要成果:
- 证实L-谷氨酸是rBCG-百日咳生长的最佳基质.
- 在pH7.4的料批培养改善了第4天后的特定生长率,减少了培养时间,尽管最大生长率没有提高.
- 与简单批次培养相比,食批次培养在冷干燥后表现出优异的可活性细胞恢复,特别是在培养后收集的样本中.
结论:
- 食批量策略,特别是pH值为7.4,在减少rBCG-百日咳的培养时间方面具有优势.
- 在食批量培养中添加L-谷氨酸似乎可以在解冷化过程中增强细胞冷保护.
- 这种优化的潜水培养方法显示出对rBCG-pertussis的更强大和标准化的生产有希望.
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