通过使用两种新型GH1β-葡萄糖酶,有效地将伊卡林生物转化为宝化物I
Xiaoling Zhang1,2,3, Yitong Wang1, Tiantian Zhang1
1School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.
Molecules (Basel, Switzerland)
|November 27, 2024
概括
研究人员从牛便微生物中发现了两种新型β-葡萄糖酶,它们可以有效地将伊卡林转化为罕见的,高度生物活性的化物I. 这些酶显示出从Epimedium Folium中产生有价值的化合物的潜力.
科学领域:
- 生物技术是生物技术.
- 酶学 是一种酶学.
- 微生物学 微生物学
背景情况:
- 叶 (Epimedium Folium,简称EF) 是一种传统的中医药,含有生物活性黄类糖化物,如icariin.
- 巴奥胡酸I是一种罕见的EF类黄,与icariin相比,它具有更高的生物活性和生物可用性.
- β-葡萄糖酶可以有效地将icariin生物转化为baohuoside I.
研究的目的:
- 从牛便微生物群中选和表征β-葡萄糖酶,以检测伊卡林生物转化为宝oside I.
- 评估有希望的β-葡萄糖酶候选物的酶活性,稳定性和结构性质.
主要方法:
- 来自牛便微生物群的33个候选β-葡萄糖酶基因的选CAZyme数据集.
- 酶测试以确定活性,最佳温度和pH值.
- 结构分析和薄层染色学以评估酶功能和产品形成.
主要成果:
- 13个基因表现出β-葡萄糖酶活性;DCF-bgl-26和DCF-bgl-27显示出高表达和活性.
- 两个酶的最佳活性是在45°C和pH值6.0时,DCF-bgl-26表现出更好的稳定性.
- DCF-bgl-26和DCF-bgl-27的活性分别为123.2U/mg和157.9U/mg,在30分钟内有效地将伊卡林转化为化物I.
结论:
- DCF-bgl-26和DCF-bgl-27是强大的β-葡萄糖酶,有可能用于工业应用.
- 这些酶可以有效地从icariin中产生高价值的baohuoside I.
- 这项研究强调了微生物酶在天然产品衍生中的潜力.
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