在生物反应器培养和选定的生物活动中对乳酸的定量分析
Kamila Goderska1, Wojciech Juzwa2, Tomasz M Karpiński3
1Department of Food Technology of Plant Origin, Faculty of Food Science and Nutrition, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, Poland.
Molecules (Basel, Switzerland)
|November 27, 2024
概括
这项研究使用Pseudomonas taetrolens细菌优化了乳酸的生产,达到37.42g/L的高产量. 这项研究还探讨了酸的益生菌和抗生素特性,成功地对Bifidobacterium bifidum进行了微封装.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 生物化学 生化学
背景情况:
- 乳清是乳制品行业的副产品,为可持续生物加工提供了机会.
- 乳酸 (LBA) 是一种有价值的化合物,在食品和制药行业具有潜在的应用.
- 优化细菌发酵以生产LBA对于其商业可行性至关重要.
研究的目的:
- 量化分析Pseudomonas taetrolens使用乳清的乳酸 (LBA) 生产情况.
- 识别和评估影响LBA产量的关键培养参数.
- 评估LBA的前生物和对抗性质.
主要方法:
- 用乳清作为介质培养Pseudomonas taetrolens的生物反应器培养.
- 优化培养参数,包括乳糖度和细菌菌株选择.
- 使用高性能液态染色学 (HPLC) 量化乳糖和LBA.
- 流细胞计与成像用于分析微生物代谢活动.
- 在体外胃肠道模型评估微封装Bifidobacteria的存活率和益生菌效应.
主要成果:
- 在优化条件下 (2%乳糖,特定的Pseudomonas taetrolens菌株) 取得的最高LBA产量为37.42g/L.
- 流细胞计提供了关于LBA发酵期间微生物代谢活动的见解.
- 用LBA微封装Bifidobacterium bifidum在模拟的胃肠道环境中证明了增强的生存率.
- 乳酸表现出对抗性活性,表明潜在的抗菌应用.
结论:
- 伪虫 (Pseudomonas taetrolens) 是一种有效的微生物,可以从乳清中生产LBA.
- 优化的生物反应器条件显著提高了LBA产量.
- 乳酸具有有希望的前生物特性,并表现出对抗性活性.
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