相关实验视频

Updated: Jun 6, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

在CRISPR基础上编辑Medicago truncatula LEC1基因

Elina A Potsenkovskaia^1,2,3, Varvara E Tvorogova^1,2,3, Veronika Y Simonova²

¹Department of Genetics and Biotechnology, Saint Petersburg State University, 7/9 Universitetskaya Embankment, 199034 Saint Petersburg, Russia.

Plants (Basel, Switzerland)

|November 27, 2024

概括

叶子COTYLEDON1 (LEC1) 基因调节了植物的发育. 失功能的Medicago truncatula MtLEC1 (MtNF-YB10) 突变体的体质胚胎发生减少,这表明LEC1的奥托洛格功能有所不同.

科学领域:

植物分子生物学植物分子生物学
发展生物学发展生物学
遗传学是一个遗传学.

背景情况:

叶子COTYLEDON1 (LEC1) 基因在Arabidopsis thaliana的发育中起着至关重要的作用,包括胚胎发生和开花.
在其他植物物种中,LEC1正向因子的功能在很大程度上仍未被描述.

研究的目的:

通过CRISPR/Cas9基因组编辑来研究Medicago truncatula LEC1正统学,MtLEC1 (MtNF-YB10) 的功能.
为了确定MtLEC1是否对于正常生长,种子活力和体质胚胎发生在体外是必不可少的.

主要方法:

在使用CRISPR/Cas9.9的Medicago truncatula中产生了MtLEC1 (MtNF-YB10) 基因的功能丧失突变.
现型特征编辑植物的生长,生育和体质胚胎生成能力.
在种子形成过程中分析了T1后代的潜在CRISPR/Cas9编辑事件.

主要成果:

MtLEC1功能丧失突变体表现出正常生长,并产生可活的种子.
实验室内体质胚胎发生在MtLEC1突变体中显著受损.
在T1世代中,从未经过编辑的母植物中没有检测到任何编辑的植物.

结论:

梅迪卡戈·特伦卡图拉MtLEC1 (MtNF-YB10) 对于植物生长或繁殖来说并不重要,但在体质胚胎发生过程中起着至关重要的作用.
这些发现表明,在植物物种中,LEC1正向因子的功能作用不同.
这项研究为探索MtNF-YB10在豆类植物中的特定功能提供了基础.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?LEC1 LEC1 的意思是什么?在医疗中,我们可以使用truncatula.在MtNF-YB10中.农业细菌转化的转化复兴再生是一种再生方式.实体胚胎发生体质胚胎发生.

更多相关视频

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins

Published on: April 30, 2018

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Published on: March 16, 2022

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...