在匈牙利山羊种群中,首次对小反动物病毒进行了分子鉴定
László Ózsvári1,2, Krisztina Bárdos1,2, Agata Moroz-Fik3
1Department of Veterinary Forensics and Economics, University of Veterinary Medicine Budapest, István u. 2, 1078 Budapest, Hungary.
Pathogens (Basel, Switzerland)
|November 27, 2024
概括
小反动物病毒 (SRLV) 感染匈牙利山羊,检测出A型和B型基因组. 这项研究揭示了匈牙利山羊群中传播的混合感染和特定亚型.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子流行病学分子流行病学
- 羊的健康 羊的健康
背景情况:
- 小反动物病毒 (SRLV) 是影响全球山羊种群的重要病原体.
- 以前的血清调查表明,匈牙利山羊群中存在SRLV.
- 了解SRLV基因型和亚型对于疾病管理和控制至关重要.
研究的目的:
- 在匈牙利山羊中分子描述SRLV基因型和亚型.
- 为了确定SRLV基因型A和B在选定的群体中的患病率.
- 调查单个和混合SRLV感染的发生情况.
主要方法:
- 收集了来自十个SRLV阳性匈牙利羊群的135只成年山羊的血液样本.
- 两阶段嵌套实时PCR (nRT-PCR) 用于检测SRLV前病毒DNA并区分A和B基因型.
- 用MEGA11软件对来自LTR和GAG区域的桑格测序数据进行了家族遗传学分析.
主要成果:
- 在所有10个研究的羊群中检测到SRLV前病毒DNA (110/135只山羊,81.5%).
- 阳性山羊中44.5% (49/110) 发现了A型,49.1% (54/110) 发现了B型,6.4% (7/110) 发现了混合感染 (A+B).
- 匈牙利SRLV分离物属于A1/A18,A2和B1亚型,在人群中确认了A和B两种基因型.
结论:
- 这项研究提供了SRLV基因型A和B在匈牙利山羊中共循环的第一个分子证据.
- 这些发现突显了匈牙利SRLV的遗传多样性,包括特定的亚型.
- 分子监测对于了解SRLV流行病学和告知山羊种群的控制策略至关重要.
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