石榴皮提取物通过MAPK和NF-κB路径调节在老鼠中减轻腹-占主导地位的刺激性肠综合征
Yannan Zhang1,2, Sijuan Huang1,2, Shuai Zhang1,2
1National Key Laboratory of Veterinary Public Health Security, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
石榴皮提取物 (PPE) 通过减少炎症和改善肠道屏障功能,有效治疗腹占主导地位的刺激性肠综合征 (IBS-D). 它通过抑制MAPK和NF-κB信号通路而起作用,为IBS-D提供了一种新的治疗方法.
科学领域:
- 胃肠病学 胃肠病学
- 药理学 药理学是指药理学的学科.
- 分子生物学分子生物学
背景情况:
- 腹占主导地位的刺激性肠综合征 (IBS-D) 是一种慢性功能性胃肠疾病,其特征是腹和肠壁功能障碍.
- 石榴皮提取物 (PPE) 显示出治疗腹和肠道炎症的潜力,但其在IBS-D中的有效性和机制尚不清楚.
研究的目的:
- 在IBS-D.的老鼠模型上研究PPE的治疗效果.
- 为了阐明个人防护品在IBS-D.中作用的潜在分子机制.
主要方法:
- 通过使用高脂肪饮食,克制压力和 senna gavage,建立了一个IBS-D大鼠模型.
- 网络药理学,血清药物化学,转录组学,分子对接和西方污点分析被用来探索机制.
- 评估了PPE对IBS-D症状,肠道炎症和屏障功能的治疗效果.
主要成果:
- 个人防护品显著缓解了IBS-D症状,减少了肠道炎症,并增强了肠道屏障功能.
- 网络药理学确定了MAPK和NF-κB信号通路作为关键目标,这些通路被PPE显著丰富和下调.
- 分子对接和Western blot证实了PPE对目标蛋白的高度亲和力及其对关键信号分子 (p-IκB,p-p65,p-JNK,p-p38,p-ERK1/2) 的显著下调调.
结论:
- 这项研究提供了第一个证据,即PPE可以缓解IBS-D的腹症状和肠道损伤.
- 个人防护设备的治疗效果很可能通过抑制MAPK和NF-κB信号通路来介导.
- 个人防护品代表了治疗IBS-D的潜在新型治疗剂.
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