压力可以诱导牛类阿尔法疹病毒1 (BoHV-1) 从潜伏状态重新激活
Fouad El-Mayet1,2, Clinton Jones1
1Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078, USA.
Viruses
|November 27, 2024
概括
牛阿尔法疹病毒1 (BoHV-1) 的重新激活是由压力引发的. 像葡萄糖皮质体受体 (GR) 和KLF4这样的先驱转录因子是启动病毒基因表达在延迟重新激活期间的关键.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 牛α-疹病毒1 (BoHV-1) 由于延迟和重新激活,导致牛牧业的重大损失.
- 再激活的BoHV-1导致生殖问题,小牛传播,免疫抑制和牛呼吸系统疾病 (BRD).
- 压力,以德克萨米他为例,可靠地诱导BoHV-1从潜伏状态的重新激活.
研究的目的:
- 调查压力诱导的BoHV-1重新激活背后的分子机制.
- 为了确定涉及到BoHV-1从延迟重新激活的早期阶段的关键转录因子.
主要方法:
- 使用德甲研究了压力诱导的BoHV-1再激活.
- 分析了葡萄糖皮质体受体 (GR),KLF15和KLF4在病毒基因表达中的作用.
- 研究了转录因子对病毒促进体的合作作用,包括IEtu1和bICP0.0.
主要成果:
- 压力介导的GR激活刺激BoHV-1复制和病毒蛋白bICP0和bICP4.4的表达.
- GR和KLF15形成了一个前循环,激活IEtu1促进体.
- GR和KLF4作为先驱转录因子,通过重塑染色体,协同激活bICP0促进体.
结论:
- 先进的转录因子GR和KLF4对于启动BoHV-1的重新激活至关重要.
- 这些因素可能会在潜伏逃逸期间调解早期染色质重塑和基因激活事件.
- 了解这些机制为控制牛群中BoHV-1的重新激活提供了潜在的目标.
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