被遗忘的托巴莫病毒基因编码54kDa蛋白质和4-6kDa蛋白质
Peter Palukaitis1, Masoud Akbarimotlagh2, Sajad Astaraki2
1Graduate School of Plant Protection and Quarantine, Jeonbuk National University, Jeonju 54896, Republic of Korea.
Viruses
|November 27, 2024
概括
本综述探讨了未表征的托巴莫病毒基因产物,包括54kDa的RNA依赖RNA聚合酶段和新型P6蛋白. 讨论了它们的潜在功能和基因组分布.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 托巴莫病毒具有未表征的基因产物,包括54kDa蛋白和小P6蛋白,其功能仍然基本未知.
- 54kDa的蛋白质是一种依赖于RNA的RNA聚合酶段,通过体外翻译确定,但在植物中没有观察到.
- 小蛋白质 (P6蛋白质) 通过运动蛋白或状蛋白质基因中的替代阅读框架来表达.
研究的目的:
- 审查有关非特征化托巴莫病毒基因产品的现有文献.
- 在45个托巴莫病毒基因组中分析新型P6蛋白的分布和分类.
- 根据现有的数据,为54kDa蛋白和P6蛋白提出功能模型.
主要方法:
- 关于托巴莫病毒基因产品的文献综述.
- 对45个托巴莫病毒基因组进行生物信息分析,以确定P6开放的读取框架 (>15个氨基酸).
- 基于基因组分布和组织的P6蛋白质的分类.
主要成果:
- 在多巴莫病毒基因组中识别和描述众多假定的P6开放式读取框架 (15-94氨基酸).
- 描述这些P6蛋白在不同托巴莫病毒物种中的分布模式.
- 将P6蛋白质根据其基因组背景分为不同的类别.
结论:
- 这项研究强调了以前被忽视的托巴莫病毒蛋白质的存在和潜在意义.
- 拟议的功能模型为54 kDa蛋白和P6蛋白在病毒生物学中的作用提供了洞察力.
- 进一步的研究是有必要的,以实验验证这些神秘的病毒蛋白的功能.
关键词:
126 kDa 的蛋白质是什么?183 kDa 的蛋白质.54 kDa 的蛋白质是54 kDa 的蛋白质.在ORF6的ORF6上.这是一种P6蛋白质.蛋白质运动 蛋白质运动烟草马赛克病毒病毒托巴莫病毒亚群 托巴莫病毒亚群托巴莫病毒病毒.番茄马赛克病毒病毒病毒蛋白的功能 病毒蛋白的功能更多相关视频
09:13Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
Published on: November 1, 2011
17.4K
11:40Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
Published on: April 17, 2020
8.6K
相关概念视频
Rab Proteins
3.9K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
3.9K
Protein Complex Assembly
10.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.6K
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Retroviruses
12.2K
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
12.2K
Non-LTR Retrotransposons
11.4K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.4K
Retrovirus Life Cycles
45.7K
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
45.7K
