相关实验视频

Updated: Jun 6, 2025

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

Published on: May 11, 2020

狂犬病病毒蛋白的分离和其核蛋白的酸化增强了它们的结合性亲和力

Euripedes de Almeida Ribeiro¹, Cédric Leyrat¹, Francine C A Gérard¹

¹Institut de Biologie Structurale, Université Grenoble Alpes, CEA, CNRS, 38000 Grenoble, France.

|November 27, 2024

概括

狂犬病病毒的蛋白 (P) 与核蛋白 (N) -RNA复合体的结合通过P二分化和N酸化得到增强. 在Ser389的酸化显著增加了P结合亲和力,这对于病毒RNA合成至关重要.

科学领域:

分子病毒学分子病毒学.
结构生物学是结构生物学.
生物化学生物化学

背景情况:

负感RNA病毒的RNA合成机器依赖于蛋白 (P) 和核蛋白 (N) -RNA复合体之间的相互作用.
已知P多元化和N酸化是调节剂,但它们在病毒RNA合成中的确切作用尚不清楚.

研究的目的:

阐明控制狂犬病病毒 (RABV) P和N-RNA复合体之间的相互作用的分子机制.
量化P二分化和N酸化对结合亲和力的影响.

主要方法:

使用表面等离子体共振 (SPR) 来测量结合相互作用.
使用了复合RABV N和P蛋白.
分析了全长P二极体和单体N-RNA片段的结合同热体.

主要成果:

RABV P对圆形和线性N-RNA复合体表现出纳米分子结合亲和力.
与单体碎片相比,P二分化增加了15-30倍的结合亲和力.
在Ser389中酸化N会产生高亲和度位点,增加P结合亲和度约360倍.

结论:

P二分化显著增强了与N-RNA复合体的结合,尽管对于双价联体来说比预测的要小.
在Ser389的N酸化是高亲和度P结合的关键因素,对于病毒RNA合成调节至关重要.

关键词:

单一无线病毒的存在.结合性亲和力是一种结合性亲和力.核蛋白质核蛋白质是一种核蛋白质.蛋白质是一种脂蛋白.酸化的方法是:酸化.狂犬病病毒病毒的病毒.表面的等离子体共振.

更多相关视频

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

Published on: December 14, 2017

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

相关概念视频

Rab Proteins

Rab Proteins

Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...

Rab Cascades

Rab Cascades

Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.