红素诱导的神经毒性:评估酵素功能障碍,氧化损伤,DNA损伤和Wistar老鼠的组织病理变化
1Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab, India.
Journal of applied toxicology : JAT
|November 27, 2024
概括
合成食品染料红色素在老鼠中引起大脑毒性,即使是低剂量. 这项研究发现,它会损害抗氧化酶,增加氧化应激,并损害大脑DNA和结构.
科学领域:
- 神经科学是一个神经科学.
- 毒理学 毒理学 毒理学
- 食品科学 食品科学 食品科学
背景情况:
- 氨酸是一种广泛使用的合成红色食品染料.
- 人们对其潜在的健康风险存在担忧.
- 它在大脑中的亚急性毒性需要彻底评估.
研究的目的:
- 评估Erythrosine在Wistar大鼠大脑中的亚急性毒性.
- 研究不同剂量的红素对大脑酶活性和氧化应激标志物的影响.
- 在暴露于红后评估DNA损伤和大脑的组织学变化.
主要方法:
- 在28天的时间里,Wistar大鼠被给予1/4 ADI,1/2 ADI和ADI的红素.
- 测量了酶活性 (CAT,SOD,GST,AchE) 和氧化应激标志物 (MDA,LDH,H2O2) 的测量结果.
- 彗星测定和光显微镜被用来评估DNA损伤和大脑组织学.
主要成果:
- 氨酸显著改变了酶活性,特别是在ADI时显著降低了AchE.
- 观察到氧化应激标志物增加和DNA损伤,特别是在ADI.
- 组织病理学检查显示大脑组织异常,包括细胞缩和真空化.
结论:
- 暴露于氨酸会破坏大脑组织病理学,并抑制关键的酶活动.
- 这种染料在老鼠大脑中诱导了脂质过氧化,DNA损伤和氧化应激.
- 在剂量达到或低于可接受的每日摄入量时,氨酸在大脑中表现出毒性.
更多相关视频
15:04A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
Published on: May 5, 2009
25.2K
08:33Development of an Alpha-synuclein Based Rat Model for Parkinson's Disease via Stereotactic Injection of a Recombinant Adeno-associated Viral Vector
Published on: February 28, 2016
13.2K
相关概念视频
01:29Drugs Affecting Neurotransmitter Synthesis
1.3K
Drugs affecting neurotransmitter synthesis can impact the adrenergic neuron and the synthesis of neurotransmitters. For example, α-methyltyrosine and carbidopa target specific enzymes involved in catecholamine synthesis. α-methyltyrosine inhibits the enzyme tyrosine hydroxylase, which converts tyrosine into dopamine. By blocking this enzyme, α-methyltyrosine reduces dopamine production and other catecholamines. Carbidopa, on the other hand, inhibits the enzyme dopa decarboxylase,...
1.3K
01:12Local Anesthetics: Adverse Effects
389
While local anesthetics are generally safe and well-tolerated, they can occasionally cause adverse effects that vary in severity. Local anesthetics can induce toxicity at two distinct levels. They can either produce local effects through direct contact with the neural elements or be absorbed into the bloodstream from the injection site, leading to systemic effects.
Once absorbed into the systemic circulation, local anesthetics can affect the organs that depend on the functioning of sodium...
Once absorbed into the systemic circulation, local anesthetics can affect the organs that depend on the functioning of sodium...
389