尼古丁和vape:具有相同特征的药物聚集在一起
Titilayomi A Otenaike1,2, Oluwabukola M Farodoye1,2, Monica M de Silva3
1Doctoral Program in Genetics and Molecular Biology, Universidade Federal do Rio Grande do Sul (UFRGS), CEP, Porto Alegre, Brazil.
果中的尼古丁和vape暴露显著缩短了寿命和行为受损. 尼古丁改变了基因表达,而两者都诱导了氧化应激和亡,揭示了毒性机制.
科学领域:
- 毒理学 毒理学 毒理学
- 行为神经科学 行为神经科学
- 遗传学 遗传学 是一个
背景情况:
- 吸烟和使用电子烟 (e-cigarette,vape) 对全球健康构成重大风险.
- 尼古丁的成性质和毒性机制,特别是来自vaping的毒性机制,仍然不完全理解.
- 黄 (Drosophila melanogaster) 为研究复杂的生物过程提供了一个有价值的模型.
研究的目的:
- 为了研究尼古丁和vape的毒理机制,使用Drosophila.
- 评估尼古丁和电子烟对行为,氧化应激,基因表达和亡的影响.
- 为了分析蒸汽液体的化学成分.
主要方法:
- 暴露于不同度的尼古丁和电子烟的Drosophila5天.
- 评估行为参数,寿命,睡眠模式和氧化应激生物标志物.
- 对多巴胺1-受体1 (Dop1R1),乙-共酶A合成酶 (AcCoAs) 和亡蛋白的mRNA表达的分析.
- 气体染色学-质谱学 (GC/MS) 和气体染色学-火焰电离检测 (GC/FID) 用于蒸汽成分分析.
主要成果:
- 尼古丁和电子烟暴露都显著减少了寿命,损害了运动活动,并扰乱了睡眠.
- 尼古丁暴露上调了Dop1R1并改变了乙-CoA基因表达.
- 较高的活性氧物种和亡标记 (Reaper,DIAP1) 表示细胞损伤.
- 蒸汽组合分析确定了二醇,尼古丁,甲基和一个未知的化合物.
结论:
- 尼古丁和vape对Drosophila产生显著的毒性影响,影响寿命和行为.
- 尼古丁会影响关键的神经递质和代谢通路,导致毒性.
- 氧化应激和亡是尼古丁和电子烟毒性的关键机制.
- 了解模型生物中的这些机制可以为有关尼古丁使用的公共卫生策略提供信息.
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